Cyanide produced by human isolates of Pseudomonas aeruginosa contributes to lethality in Drosophila melanogaster
Cyanide produced by human isolates of Pseudomonas aeruginosa contributes to lethality in Drosophila melanogaster
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DOI：
10.1086/525282
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发表时间：
2008-02-01
影响因子：
6.4
通讯作者：
Boss, Gerry R.
中科院分区：
文献类型：
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作者：
Broderick, Kate E.;Chan, Adriano;Boss, Gerry R.
一些铜绿假单胞菌菌株是产氰的，氰化物可能有助于细菌的毒力。使用铜绿假单胞菌的人类分离株，我们已经表明，果蝇悬浮在生氰菌株变得不动，并发展心动过缓，果蝇注射生氰菌株比注射非生氰菌株更快地死亡。暴露于生氰菌株的苍蝇有高氰化物和低三磷酸腺苷（ATP）浓度的身体提取物，和治疗与氰化物解毒剂均衡的苍蝇注射生氰和noncyanogenic菌株的生存。铜绿假单胞菌PAO 1菌株与一个突变的氰化氢合酶基因簇对苍蝇的毒性远低于亲氰菌株或2敲入菌株。转基因果蝇过度表达罗丹酸，通过将其转化为硫氰酸盐来解毒氰化物，对氰化物具有抵抗力，并增加了产氰菌株的毒力。我们的结论是D.黑腹菌是研究铜绿假单胞菌产生氰化物的良好模型。
Some Pseudomonas aeruginosa strains are cyanogenic, and cyanide may contribute to the bacterium's virulence. Using human isolates of P. aeruginosa, we have shown that Drosophila melanogaster suspended above cyanogenic strains become motionless and develop bradycardia and that flies injected with cyanogenic bacterial strains die more rapidly than those injected with noncyanogenic strains. Flies exposed to cyanogenic strains had high cyanide and low adenosine triphosphate (ATP) concentrations in body extracts, and treatment with a cyanide antidote equalized survival of flies injected with cyanogenic and noncyanogenic strains. P. aeruginosa PAO1 strain with a mutation in the hydrogen cyanide synthase gene cluster was much less toxic to flies than the parental cyanogenic strain or 2 knock-in strains. Transgenic flies overexpressing rhodanese, which detoxifies cyanide by converting it to thiocyanate, were resistant to cyanide and the increased virulence of cyanogenic strains. We conclude that D. melanogaster is a good model for studying cyanide produced by P. aeruginosa.