Release of prostaglandin E2 from cells by photodynamic treatment in vitro.
Release of prostaglandin E2 from cells by photodynamic treatment in vitro.
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DOI：
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发表时间：
1989-12
期刊：
影响因子：
11.2
通讯作者：
B. Henderson;Jean M. Donovan
中科院分区：
文献类型：
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作者：
B. Henderson;Jean M. Donovan
在体外光动力治疗，使用光敏剂Photofrin II和光在630 nm，被发现释放大量的前列腺素E2（PGE2）从小鼠辐射诱导的纤维肉瘤肿瘤细胞和腹腔巨噬细胞，但不是从L929成纤维细胞。PGE2释放呈剂量依赖性，并与细胞膜破裂直接相关。它发生迅速，并在治疗后30分钟内完成。PGE2的释放可被吲哚美辛、甲氨蝶呤和地塞米松的长期暴露所抑制，表明这是由于涉及磷脂酶和环氧合酶系统的新产物。从培养基中去除磷脂酶激活所必需的钙离子并不能抑制光动力学诱导的PGE2产量升高，这可能是因为细胞内钙离子的再供应。
Photodynamic treatment in vitro, using the photosensitizer Photofrin II and light at 630 nm, was found to liberate large amounts of prostaglandin E2 (PGE2) from mouse radiation-induced fibrosarcoma tumor cells and peritoneal macrophages, but not from L929 fibroblasts. PGE2 release was dose dependent and directly related to cell membrane disruption. It occurred rapidly and was complete within 30 min of treatment. PGE2 release could be inhibited by indomethacin, meclofenamate and extended prior exposure to dexamethasone, indicating that it was due to new production involving both the phospholipase and cyclooxygenase enzyme systems. Removal of calcium ions, necessary for phospholipase activation, from the medium did not inhibit the photodynamically induced elevated PGE2 production, possibly because of Ca2+ resupply from leaking intracellular pools.