Mutations in the ATP-binding domain affect the subcellular distribution of mitotic centromere-associated kinesin (MCAK).
Mutations in the ATP-binding domain affect the subcellular distribution of mitotic centromere-associated kinesin (MCAK).
复制标题
ATP 结合域的突变会影响有丝分裂着丝粒相关驱动蛋白 (MCAK) 的亚细胞分布。
DOI：
10.1006/cbir.1999.0359
复制
发表时间：
1999
影响因子：
3.9
通讯作者：
Maney,T
中科院分区：
文献类型：
--
作者：
Wordeman,L;Wagenbach,M;Maney,T
有丝分裂着丝粒相关驱动蛋白（MCAK）对后期染色体分离很重要。MCAK在间期广泛定位于细胞质和细胞核。在前期，MCAK被募集到有丝分裂的着丝粒。在整个有丝分裂过程中，它与着丝粒相关，然后在间期恢复到弥漫性的核和细胞质定位。MCAK有几个预测的核定位序列。表达的GFP-MCAK缺失构建体的亚细胞分布表明，MCAK蛋白的核质比取决于MCAK氨基末端区域中几个预测的核定位序列（NLS）和一个推定的核排除序列（内斯）之间的平衡。MCAK马达的ATP结合结构域中的氨基酸取代影响核定位，这反过来又影响着丝粒结合的程度。
Mitotic centromere‐associated kinesin (MCAK) is important for anaphase chromosome segregation. MCAK is diffusely localized to both the cytoplasm and the nucleus during interphase. At prophase MCAK is recruited to mitotic centromeres. It is associated with centromeres throughout mitosis and then returns to exhibiting a diffuse nuclear and cytoplasmic localization during interphase. MCAK has several predicted nuclear localization sequences. The subcelluar distribution of expressed deletion constructs of GFP‐MCAK suggest that the nucleocytoplasmic ratio of MCAK protein is dependent on a balance between several predicted nuclear localization sequences (NLS) and a putative nuclear exclusion sequence (NES) in the amino‐terminal region of MCAK. Amino acid substitutions in the ATP‐binding domain of the MCAK motor affect nuclear localization, which, in turn, influences the degree of centromere binding.