Characterization of H5N1 influenza viruses that continue to circulate in geese in southeastern China
Characterization of H5N1 influenza viruses that continue to circulate in geese in southeastern China
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DOI：
10.1128/jvi.76.1.118-126.2002
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发表时间：
2002-01-01
影响因子：
5.4
通讯作者：
Shortridge, KF
中科院分区：
文献类型：
--
作者：
Webster, RG;Guan, Y;Shortridge, KF
1997 年导致人类和家禽死亡的 H5N1 流感病毒是一种重组病毒，可能出现在香港活禽市场上的一种家禽中。鉴于H5N1/97的所有前体仍在中国南方的家禽中传播，产生H5N1的重组事件可能会重复。由于A/goose/Guangdong/1/96-like (H5N1; Go/Gd)病毒是H5N1病毒血凝素基因的拟议供体，因此我们研究了Go/Gd-like病毒在家禽中的持续传播、宿主范围和传播性。 Go/Gd 样病毒导致一些接种高剂量病毒的小鼠体重减轻和死亡。通过与受感染的鹅接触，Go/Gd 样 H5N1 病毒传播给鹅，导致所有鸟类都受到感染，但明显疾病的迹象有限。相比之下，口服高剂量的 Go/Gd 类病毒导致高达 50% 的受感染鹅死亡。从受感染的鹅到鸡的传播仅通过粪便接触发生，而到鹌鹑的传播则通过气溶胶或粪便传播发生。这种差异可能是由于鹌鹑对 Go/Gd 样病毒的易感性较高所致。鹌鹑对 Go/Gd (H5N1) 类病毒的高度易感性以及 H6N1 和 H9N2 病毒在鹌鹑中的持续传播支持了鹌鹑是 H5N1/97 病毒起源宿主的假设。 Go/Gd (H5N1) 类病毒很容易传播给陆生鸟类，尤其是鹌鹑，这支持了在香港市场上区分水生家禽和陆生家禽的明智做法，以及对不同类型家禽相互接触的现场和活禽市场进行持续监测的需要。
The H5N1 influenza virus, which killed humans and poultry in 1997, was a reassortant that possibly arose in one type of domestic poultry present in the live-poultry markets of Hong Kong. Given that all the precursors of H5N1/97 are still circulating in poultry in southern China, the reassortment event that generated H5N1 could be repeated. Because A/goose/Guangdong/1/96-like (H5N1; Go/Gd) viruses are the proposed donors of the hemagglutinin gene of the H5N1 virus, we investigated the continued circulation, host range, and transmissibility of Go/Gd-like viruses in poultry. The Go/Gd-like viruses caused weight loss and death in some mice inoculated with high virus doses. Transmission of Go/Gd-like H5N1 viruses to geese by contact with infected geese resulted in infection of all birds but limited signs of overt disease. In contrast, oral inoculation with high doses of Go/Gd-like viruses resulted in the deaths of up to 50% of infected geese. Transmission from infected geese to chickens occurred only by fecal contact, whereas transmission to quail occurred by either aerosol or fecal spread. This difference is probably explained by the higher susceptibility of quail to Go/Gd-like virus. The high degree of susceptibility of quail to Go/Gd (H5N1)-like viruses and the continued circulation of H6N1 and H9N2 viruses in quail support the hypothesis that quail were the host of origin of the H5N1/97 virus. The ease of transmission of Go/Gd (H5N1)-like viruses to land-based birds, especially quail, supports the wisdom of separating aquatic and land-based poultry in the markets in Hong Kong and the need for continued surveillance in the field and live-bird markets in which different types of poultry are in contact with one another.