Discovery and antitumor activities of constituents from Cyrtomium fortumei (J.) Smith rhizomes.
Discovery and antitumor activities of constituents from Cyrtomium fortumei (J.) Smith rhizomes.
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贯众根茎成分的发现及其抗肿瘤活性
DOI：
10.1186/1752-153x-7-24
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发表时间：
2013-02-04
影响因子：
--
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Yang S;Liu M;Liang N;Zhao Q;Zhang Y;Xue W;Yang S
研究背景贯众Cyrtomium fortumei（J.）史密斯是一个重要的中草药，因为它的生物功能。然而，目前对贯众植物化学成分的系统、全面的研究尚不多见。结果采用生物活性导向技术，对贯众根茎的乙酸乙酯和正丁醇提取物进行了活性鉴定。Smith的新化合物具有良好的抗肿瘤活性，从中分离得到23个化合物。所有化合物均为首次从该植物中分离得到。采用MTT法测定了化合物对MGC-803、PC 3和A375细胞的体外抑制活性结果表明，茴芹素（3）对上述3种细胞株均具有较强的细胞毒活性，IC_（50）分别为14.4 ± 0.3 μM、20.4 ± 0.5 μM和29.2 ± 0.6 μM，分别20 μM的茴芹素处理MGC-803细胞72 h后，细胞凋亡率为27.44%。结论该研究表明，从贯众根中提取的化合物中，大部分是通过诱导肿瘤细胞凋亡来抑制MGC-803细胞生长的。史密斯可以抑制人类癌细胞的生长。此外，茴芹素通过诱导肿瘤细胞凋亡抑制肿瘤细胞的生长。
BackgroundCyrtomium fortumei (J.) Smith is an important Chinese herbal medicine because of its biological functions. However, systematic and comprehensive studies on the phytochemicals from Cyrtomium fortumei (J.) Smith and their bioactivity are limited.ResultsUsing the bioassay-guided technique, the ethyl acetate and n-BuOH extracts of the rhizomes of Cyrtomium fortumei (J.) Smith were shown to exhibit good antitumor activities, consequently leading to the isolation of 23 compounds. All compounds were isolated from the plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells MGC-803, PC3, and A375 in vitro by MTT (thiazolyl blue tetrazolium bromide) assay, and the results showed that pimpinellin (3) had potent cytotoxic activities against the three cell lines, with the IC50 values of 14.4 ± 0.3 μM, 20.4 ± 0.5 μM, and 29.2 ± 0.6 μM, respectively. The mechanism of the antitumor action indicated that pimpinellin inhibited the growth of MGC-803 cells via the induction of tumor cell apoptosis, with apoptosis ratio of 27.44% after 72 h of treatment at 20 μM.ConclusionsThis study suggests that most of the compounds from the roots of Cyrtomium fortumei (J.) Smith could inhibit the growth of human carcinoma cells. Moreover, pimpinellin inhibited the growth of tumor cells via the induction of tumor cell apoptosis.