Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3
Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3
复制标题
富含三萜的灵芝提取物通过激活 caspase-3 诱导 SW620 结肠癌细胞系凋亡
DOI：
10.3892/ol.2011.275
复制
发表时间：
2011-05-01
期刊：
影响因子：
2.9
通讯作者：
Pan, Yingje
中科院分区：
文献类型：
--
作者：
Ji, Zhe;Tang, Qingju;Pan, Yingje
药用蘑菇灵芝（G. lucidum）已用于治疗多种疾病，并以其多糖的免疫增强活性而闻名。然而，人们对其另一种主要成分三萜知之甚少。本研究调查了灵芝中富含三萜的提取物的抗癌机制。富含三萜的提取物GLAI是以灵芝子实体为原料，通过连续热水提取、去除乙醇不溶性多糖和凝胶过滤层析制备的。研究了 GLAI 诱导 SW620 人结直肠腺癌细胞凋亡的机制。用不同浓度的GLAI处理体外肿瘤细胞系。 Alamar蓝法检测细胞增殖，观察细胞凋亡形态，流式细胞仪（FCM）检测细胞凋亡，Caspase-3细胞活性测定检测caspase-3活性。结果表明，GLAI以剂量依赖性方式抑制不同肿瘤细胞的生长并引起显着的细胞凋亡。 GLAI作用24 h后，细胞出现明显的细胞凋亡形态学变化。 Caspase-3检测结果表明，caspase-3酶的活性以时间和剂量依赖性方式增加，而GLAI导致mRNA水平上的Bcl-2基因表达下调和蛋白质水平上的XIAP蛋白产生下调。相反，GLAI 上调凋亡增强子 Bax 基因和 p53 蛋白的表达。这些发现表明灵芝中含有的三萜是潜在的抗癌剂。
The medicinal mushroom Ganoderma lucidum (G. lucidum) has been used for the treatment of various diseases, and is known for the immune-enhancing activity of its polysaccharide. However, little is known about another of its major constituents, triterpene. This study investigated the anticancer mechanism of a triterpene-enriched extract from G. lucidum. The triterpene-enriched extract, GLAI, was prepared from fruiting bodies of G. lucidum by sequential hot water extraction, removal of ethanol-insoluble polysaccharides and gel-filtration chromatography. The mechanisms of GLAI-induced apoptosis on SW620 human colorectal adenocarcinoma cells were investigated. Tumor cell lines in vitro were treated with different concentrations of GLAI. Cell proliferation was measured by the Alamar blue assay, morphology of cell apoptosis was observed, cell apoptosis was detected by flow cytometry (FCM) and caspase-3 activity was detected by Caspase-3 cellular activity assay. The results showed that GLAI inhibited the growth of different tumor cells and caused significant apoptosis in a dose-dependent manner. Marked morphological changes of cell apoptosis were observed after the cells had been exposed to GLAI for 24 h. The Caspase-3 assay results showed that the activity of the caspase-3 enzyme increased in both a time- and dose-dependent manner, whereas GLAI resulted in the down-regulation of Bcl-2 gene expression at the mRNA level and XIAP protein production at the protein level. Conversely, GLAI up-regulates the expression of the apoptosis enhancer Bax gene and p53 protein. These findings suggest that the triterpenes contained in G. lucidum are potential anticancer agents.