Soluble CD86 is a costimulatory molecule for human T lymphocytes
Soluble CD86 is a costimulatory molecule for human T lymphocytes
复制标题
DOI：
10.1016/s1074-7613(00)00030-3
复制
发表时间：
2000-09-01
期刊：
影响因子：
32.4
通讯作者：
Delneste, Y
中科院分区：
文献类型：
--
作者：
Jeannin, P;Magistrelli, G;Delneste, Y
CD86是启动和激活幼稚T细胞和记忆T细胞的重要共刺激分子。在这里，我们发现在人血清中检测到可溶性CD86。可溶性CD86是由静息的单核细胞产生的，由一个选择性剪接的转录本(CD86 Delta TM)产生，其特征是跨膜区的缺失。重组CD86 Delta TM可与CD28和CTLA-4结合，并在抗CDS单抗刺激下诱导T细胞活化。CD86 Delta TM还通过流感M1肽刺激的病毒特异性CD8(+)记忆性人类T细胞诱导产生干扰素。在人血清中发现的可溶性CD86浓度足以诱导生物活性。因此，可溶性CD86分子似乎是一种功能性的共刺激分子，在免疫监测中发挥着潜在的重要作用。
CD86 is an important costimulatory molecule for the priming and activation of naive and memory T cells, respectively. Here, we show that soluble CD86 is detected in human serum. Soluble CD86 is produced by resting monocytes and results from an alternatively spliced transcript (CD86 Delta TM) characterized by deletion of the transmembrane domain. Recombinant CD86 Delta TM binds to CD28 and CTLA-4 and induces the activation of T cells after stimulation with anti-CDS mAb. CD86 Delta TM also induces IFN gamma production by virus-specific CD8(+) memory human T cells stimulated with the Flu M1 peptide. The concentrations of soluble CD86 found in human serum are sufficient to induce biological activity. Soluble CD86 molecule, therefore, appears to be a functional costimulatory molecule playing a potentially important role in immune surveillance.