Effects of thio-group modifications on the ion permeability control and ligand binding properties of Torpedo californica acetylcholine receptor.
Effects of thio-group modifications on the ion permeability control and ligand binding properties of Torpedo californica acetylcholine receptor.
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硫基修饰对加州鱼雷乙酰胆碱受体离子渗透性控制和配体结合特性的影响。
DOI：
10.1021/bi00511a018
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
McNamee,MG
中科院分区：
文献类型：
--
作者：
Walker,JW;Lukas,RJ;McNamee,MG
Jeffery W. Walker, Ronald J. Lukas,* and Mark G. McNamee* abstract: Chemical modification of membrane-bound Torpedo californica acetylcholine receptor by the disulfide reducing agent dithiothreitol has two major effects on receptor function:(1) it shifts the dose-response curve for agonistinduced increases in 22Na+ permeability to 10-fold higher concentrations, and (2) it decreases the binding affinity of the receptor for the same agonist about 6-fold. In the experiments reported here, the agonist used was carbamoylcholine. Despite the quantitative changes in agonist binding and flux response, dithiothreitol-treated membranes display all other functional properties expected of the receptor. The flux response is blocked by preincubation of the membranes with carbamoylcholine, a phenomenon known as desensitization. In parallel, the receptor undergoes a carbamoylcholine-induced shift from a low-affinity to a high-affinity binding state for the same agonist. All of the effects of dithiothreitol are re-versed by theoxidizing agent 5, 5/-dithiobis (2-nitrobenzoic
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鱼雷突触后膜[3H]筒箭毒碱和[3H]乙酰胆碱的平衡结合：化学计量和配体相互作用。
影响因子：
2.9
作者：
R. Neubig;Jonathan B. Cohen
通讯作者：
Jonathan B. Cohen
加州鱼雷乙酰胆碱受体在两个位点的独立激活。
影响因子：
2.9
作者：
A. Delegeane;M. McNamee
通讯作者：
M. McNamee
乙酰胆碱受体：二硫键还原剂处理后突触门控机制的改变
DOI：
10.1007/bf00584796
发表时间：
1975
期刊：
Pflügers Archiv
影响因子：
--
作者：
D. Ben;F. Dreyer;K. Peper
通讯作者：
K. Peper
影响因子：
2.9
作者：
L. Holland;B. Mccarthy
通讯作者：
B. Mccarthy
平面脂质双层中具有功能性离子通道的纯化乙酰胆碱受体的重建。
DOI：
--
发表时间：
1980
影响因子：
11.1
作者：
N. Nelson;R. Anholt;J. Lindstrom;M. Montal
通讯作者：
M. Montal