Chemical composition of various Ephedra species
Chemical composition of various Ephedra species
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DOI：
10.17305/bjbms.2015.539
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发表时间：
2015-01-01
影响因子：
3.4
通讯作者：
Sofic, Emin
中科院分区：
文献类型：
--
作者：
Ibragic, Saida;Sofic, Emin
麻黄的药用价值是基于麻黄碱(E)生物碱的拟交感神经特性。药理作用取决于个别麻黄物种的植物组成。本研究旨在测定大麻黄干草本植物中总生物碱（TAC）、总酚类物质（TPC）和总黄酮（TFC）含量，并确定三者之间的关系。麻黄，单精子麻黄，麻黄，黄麻黄，黄麻黄，黄麻黄，黄麻黄，黄麻黄。目前，麻黄的药用范围有限，但滥用麻黄类精神兴奋剂的现象却呈上升趋势。本研究采用分光光度法测定TAC、TPC和TFC。首次采用超高效液相色谱-紫外检测（UPLC-UV）技术对麻黄中e型生物碱进行了分离和定量。TPC和TFC含量最高的是白莲，分别为53.3 +/- 0.1 mg没食子酸当量/g干重和2.8 mg槲皮素当量/g干重。UPLC-UV法测定的E和伪麻黄碱总含量在20.8 mg/g干重(E. distachya subsp.；干重34.7 mg/g （E. monosperma）。次生代谢物含量和比值的变化反映了麻黄属植物的代谢活性。UPLC-UV的应用表明，该技术具有较高的灵敏度和选择性，可用于复杂生物基质中不同生物碱的分离和定量。检出限为5 ng。UPLC-UV可用于法医学中e型生物碱的快速分析和药物制剂的质量控制。
The medicinal significance of Ephedra is based on the sympathomimetic properties of ephedrine (E) alkaloids. Pharmacological effects depend on the phytocomposition of individual Ephedra species. The aim of this study was to measure the total alkaloids content (TAC), total phenolics content (TPC), and total flavonoids content (TFC) and determine their relationship in dry herb of Ephedra major, Ephedra distachya subsp. helvetica, Ephedra monosperma, Ephedra fragilis, Ephedra foeminea, Ephedra alata, Ephedra altissima and Ephedra foliata. Nowadays, medicinal use of Ephedrae herba is limited, but the abuse of its psychostimulants is rising. In this study, TAC, TPC and TFC were determined using spectrophotometric methods. For the first time, ultra-performance liquid chromatography with ultraviolet detection (UPLC-UV) was used for separation and quantification of E-type alkaloids of various Ephedra species. The highest TPC and TFC were found in E. alata (53.3 +/- 0.1 mg Gallic acid equivalents/g dry weight, 2.8 mg quercetin equivalents/g dry weight, respectively). The total content of E and pseudoephedrine determined by UPLC-UV varied between 20.8 mg/g dry weight (E. distachya subsp. helvetica) and 34.7 mg/g dry weight (E. monosperma). The variable content and ratio between secondary metabolites determined in different Ephedra species reflects their metabolic activities. Utilization of UPLC-UV unveiled that this technique is sensitive, selective, and useful for separation and quantification of different alkaloids in complex biological matrixes. The limit of detection was 5 ng. Application of UPLC-UV can be recommended in quick analyses of E-type alkaloids in forensic medicine and quality control of pharmaceutical preparations.