Altered antioxidant status in peripheral skeletal muscle of patients with COPD
Altered antioxidant status in peripheral skeletal muscle of patients with COPD
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DOI：
10.1016/j.rmed.2004.05.018
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发表时间：
2005-01-01
影响因子：
4.3
通讯作者：
Schols, AMWJ
中科院分区：
文献类型：
--
作者：
Gosker, HR;Bast, A;Schols, AMWJ
尽管肺氧化应激在慢性阻塞性肺疾病（COPD）中的作用越来越受到关注，但几乎没有任何关于这些患者外周肌肉组织抗氧化能力的数据。本研究的主要目的是详细评估COPD患者骨骼肌的抗氧化状态。对21例COPD患者和12例年龄匹配的健康对照者的股外侧肌活检进行了分析。总抗氧化能力，维生素E，谷胱甘肽，尿酸水平进行了测定和超氧化物歧化酶，谷胱甘肽还原酶，谷胱甘肽过氧化物酶，谷胱甘肽-S-转移酶的酶活性进行了测定。测定丙二醛作为脂质过氧化反应的指标。COPD患者总抗氧化能力和尿酸水平显著高于健康对照组（分别为25%，P = 0.006和24%，P = 0.029）。与健康受试者相比，患者的谷胱甘肽-S-转移酶活性也增加（35%; P = 0.044）。维生素E水平低于对照组（P
Despite the growing field of interest in the role of pulmonary oxidative stress in chronic obstructive pulmonary disease (COPD), barely any data are available with respect to antioxidant capacity in the peripheral musculature of these patients. The main objective of this study was to assess in detail the antioxidant status in skeletal muscle of patients with COPD. Biopsies from the vastus lateralis of 21 patients with COPD and 12 healthy age-matched controls were analysed. Total antioxidant capacity, vitamin E, glutathione, and uric acid levels were determined and the enzyme activities of superoxide dismutase, glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were measured. Malondialdehyde was measured as an index of lipid peroxidation. The total antioxidant capacity and the uric acid levels were markedly higher in COPD patients than in healthy controls (25%, P = 0.006 and 24%, P = 0.029, respectively). Glutathione-S-transferase activity was also increased (35%; P = 0.044) in patients compared to healthy subjects. Vitamin E level was lower in patients than in controls (P