Biosynthesis of cell wall mannan in the conidium and the mycelium of Aspergillus fumigatus
Biosynthesis of cell wall mannan in the conidium and the mycelium of Aspergillus fumigatus
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DOI：
10.1111/cmi.12665
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发表时间：
2016-12-01
影响因子：
3.4
通讯作者：
Latge, Jean-Paul
中科院分区：
文献类型：
--
作者：
Henry, Christine;Fontaine, Thierry;Latge, Jean-Paul
半乳甘露聚糖是烟曲霉的主要细胞壁分子。该分子由线性甘露聚糖组成，其重复单元由四个1，6和1，2连接的甘露糖组成，侧链为半乳呋喃。为了更好地了解烟曲霉菌中甘露聚糖的生物合成，决定对负责在酵母中建立1，6和1，2甘露糖链的甘露糖转移酶的11个基因进行连续缺失。这些缺失并未导致烟曲霉菌丝体细胞壁甘露聚糖含量的降低。相反，分生孢子细胞壁的甘露聚糖含量减少，这种减少与细胞壁的部分解体有关，从而导致分生孢子在体外和活体中存活的缺陷。这篇文章是由Neil Gow和Elizabeth Hartland编辑的关于真菌细胞壁的特刊的一部分。
The galactomannan is a major cell wall molecule of Aspergillus fumigatus. This molecule is composed of a linear mannan with a repeating unit composed of four 1,6 and 1,2 linked mannose with side chains of galactofuran. To obtain a better understanding of the mannan biosynthesis in A.fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing 1,6 and 1,2 mannose linkages in yeast. These deletions did not lead to a reduction of the mannan content of the cell wall of the mycelium of A.fumigatus. In contrast, the mannan content of the conidial cell wall was reduced and this reduction was associated with a partial disorganization of the cell wall leading to defects in conidial survival both in vitro and in vivo.This article is part of a special issue on Fungal Cell Wall, edited by Neil Gow and Elizabeth Hartland.