Correlation with larval body size of mRNA levels of growth hormone, growth hormone receptor I and insulin-like growth factor I in larval torafugu Takifugu rubripes.
Correlation with larval body size of mRNA levels of growth hormone, growth hormone receptor I and insulin-like growth factor I in larval torafugu Takifugu rubripes.
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DOI：
10.1111/j.1095-8649.2011.03037.x
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发表时间：
2011-10
影响因子：
2
通讯作者：
Gen Kaneko;Satoshi Furukawa;Y. Kurosu;T. Yamada;H. Takeshima;M. Nishida;Toru Mitsuboshi;Otaka Ta
中科院分区：
文献类型：
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作者：
Gen Kaneko;Satoshi Furukawa;Y. Kurosu;T. Yamada;H. Takeshima;M. Nishida;Toru Mitsuboshi;Otaka Ta
本研究克隆了红鳍东方鲀（Takifugu rubripes）胰岛素样生长因子（insulin-like growth factor，IGF）的igf-I和igf-II基因的全长互补序列。这两个基因的氨基酸序列均为c。与其它硬骨鱼类的Igf-I和Igf-II的同源性分别为80%。两个生长激素（GH）受体，ghr 1和ghr 2，也在电子克隆使用T。红鳍东方鲀基因组数据库。T.在成鱼的慢肌、心脏、皮肤、鳃、肝和肠中检测到igf-I，而在快肌、脾和精巢中未检测到igf-II，而在所检测的所有组织中均检测到igf-II。随后，在从一个明显的快速生长的T的雄性衍生的F（2）群体中研究igf-I和igf-II的累积信使（m）RNA水平。rubripes菌株和野生雌性T. rubripes与其他生长相关基因Gh、Ghr 1和Ghr 2以及先前报道的催乳素（Prl）和瘦素（Lep）的基因一起。igf-I、GH和GHR 1的mRNA积累量与幼虫期的生长速率显著相关，而igf-II、prl、GHR 2和lep的mRNA积累量与幼虫期的生长速率不相关。虽然目前还不清楚这种表型是否与快速生长菌株的遗传直接相关，但研究结果表明，igf-I，gh和ghr 1的表达参与了T.红鳍
The full-length of insulin-like growth factor (IGF) complementary (c)DNAs encoded by igf-I and igf-II from torafugu pufferfish Takifugu rubripes were cloned in the present study. The deduced amino acid sequences of the two genes showed c. 80% identity each with those of Igf-I and Igf-II from other teleosts, respectively. Two growth hormone (GH) receptors, ghr1 and ghr2, were also cloned in silico using the T. rubripes Fugu genome database. The transcripts of T. rubripes igf-I were detected in slow muscle, heart, skin, gill, liver and intestine but not in fast muscle, spleen and testis of adult fish, whereas those of igf-II were found in all tissues examined. Subsequently, the accumulated messenger (m)RNA levels of igf-I and igf-II were investigated in an F(2) population derived from a male of an apparent fast-growing T. rubripes strain and a wild female T. rubripes together with those of other growth-related genes encoding Gh, Ghr1 and Ghr2, and with those of prolactin (Prl) and leptin (Lep) previously reported. The accumulated mRNA levels of igf-I, gh and ghr1 were significantly correlated to growth rate at larval stages in the population, but not for those of igf-II, prl, ghr2 and lep. Although it is unclear whether or not this phenotype is directly related to the heredity of the fast-growing strain, the findings suggest that the expression of igf-I, gh and ghr1 is involved in the regulation of growth rate at larval stages in T. rubripes.