Stress triggers a process that limits activation of the Bacillus subtilis stress transcription factor sigma(B).
Stress triggers a process that limits activation of the Bacillus subtilis stress transcription factor sigma(B).
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压力会触发限制枯草芽孢杆菌应激转录因子 sigma(B) 激活的过程。
DOI：
10.1128/jb.182.5.1452-1456.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
Haldenwang,WG
中科院分区：
文献类型：
--
作者：
Scott,JM;Mitchell,T;Haldenwang,WG
压力诱导的枯草芽孢杆菌转录因子β B的激活是短暂的。为了确定限制B激活的过程本身是否由压力触发，B。将通过诱导表达阳性调节蛋白（RsbT）来人工激活应激途径的枯草杆菌菌株暴露于乙醇应激，并监测其是否持续存在抗氧化活性。在无乙醇处理的情况下，诱导的培养物显示出持续的高活性。乙醇处理限制了正在进行的β-内酰胺酶活性，但仅限于具有完整trsbX和-S基因的菌株。已知参与应激激活途径的其他基因产物（RsbR和Obg）的丢失对阻断乙醇效应的影响很小。这些数据表明，应激上调RsbX-S调节对的活性，以限制应激后的RsbB诱导。
Stress-induced activation of theBacillus subtilistranscription factor ςBis transitory. To determine whether the process that limits ςBactivation is itself triggered by stress,B. subtilisstrains in which the stress pathway was artificially activated by the induced expression of a positive regulatory protein (RsbT) were exposed to ethanol stress and were monitored for the persistence of ςBactivity. Without ethanol treatment, the induced cultures displayed continuously high ςBactivity. Ethanol treatment restricted ongoing ςBactivity, but only in strains with intactrsbXand -Sgenes. The loss of other gene products (RsbR and Obg) known to participate in the stress activation pathway had little influence in blocking the ethanol effect. The data argue that stress upregulates the activity of the RsbX-S regulatory pair to restrict ςBinduction following stress.