BUTANOL PRODUCTION BY A BUTANOL-TOLERANT STRAIN OF CLOSTRIDIUM-ACETOBUTYLICUM IN EXTRUDED CORN BROTH
BUTANOL PRODUCTION BY A BUTANOL-TOLERANT STRAIN OF CLOSTRIDIUM-ACETOBUTYLICUM IN EXTRUDED CORN BROTH
复制标题
DOI：
10.1128/aem.45.3.966-973.1983
复制
发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
BLASCHEK, HP
中科院分区：
文献类型：
--
作者：
LIN, YL;BLASCHEK, HP
通过连续富集，获得了C.获得了在阻止野生型菌株生长的丁醇浓度下生长的丙酮丁醇菌ATCC 824。亲本菌株在15 g丁醇/l下表现出负生长速率; SA-1突变体仍然能够以未抑制对照的66%的速率生长。SA-1在4-20%挤压玉米肉汤（ECB）中始终产生比野生型菌株更高浓度的丁醇（5-14%）和更低浓度的丙酮（12.5-40%）。尽管SA-1和野生型菌株在14%ECB中产生最高浓度的丁醇，但对于SA-1而言，关于优化丁醇和减少丙酮的最佳溶剂比出现在4- 8%ECB之间。SA-1在每个测试的ECB浓度下都表现出比野生型菌株更高的丁醇转化效率。野生型和SA-1菌株在6%ECB中的表征证明了后者在生长速率、丁醇产生的开始时间、碳水化合物利用、pH抗性和发酵液中的最终丁醇浓度方面的优越性。
By employing serial enrichment, a derivative of C. acetobutylicum ATCC 824 was obtained which grew at concentrations of butanol that prevented growth of the wild-type strain. The parent strain demonstrated a negative growth rate at 15 g of butanol/l; the SA-1 mutant was still able to grow at a rate which was 66% of the uninhibited control. SA-1 produced consistently higher concentrations of butanol (5-14%) and lower concentrations of acetone (12.5-40%) than the wild-type strain in 4-20% extruded corn broth (ECB). Although the highest concentration of butanol was produced by SA-1 and the wild-type strain in 14% ECB, the best solvent ratio with respect to optimizing butanol and decreasing acetone occurred between 4-8% ECB for SA-1. SA-1 demonstrated higher conversion efficiency to butanol than the wild-type strain at every concentration of ECB tested. Characterization of the wild-type and SA-1 strain in 6% ECB demonstrated the superiority of the latter in terms of growth rate, time of onset of butanol production, carbohydrate utilization, pH resistance and final butanol concentration in the fermentation broth.