Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella.
Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella.
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DOI：
10.1242/bio.026278
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发表时间：
2017-11-15
期刊：
影响因子：
2.4
通讯作者：
Fan ZC
中科院分区：
文献类型：
--
作者：
Dong B;Wu S;Wang J;Liu YX;Peng Z;Meng DM;Huang K;Wu M;Fan ZC
鞭毛内运输 (IFT) 颗粒由多蛋白复合物 IFT-A 和 IFT-B 以及货物适配器（如 BBSome）组成。两个 IFT-B 亚基 IFT25 和 IFT27 被发现形成异二聚体，这对于将 BBSome 输出纤毛至关重要，但不参与脊椎动物的鞭毛组装和胞质分裂。然而，有争议的结果表明，莱茵衣藻中 IFT、鞭毛组装甚至胞质分裂的缺陷是由 IFT27 敲低引起的。使用莱茵衣藻作为模式生物，我们报告IFT25的耗尽对鞭毛组装没有影响，并且不影响BBSome进入鞭毛，但IFT25的耗尽确实损害了BBSome从鞭毛的运动，阐明了IFT25在调节BBSome从鞭毛交叉物种中退出的进化保守作用。有趣的是，正如预期的那样，IFT25 的耗尽会导致 IFT27 急剧减少，但这不会导致莱茵衣藻鞭毛组装和胞质分裂的缺陷。因此，我们的数据支持衣藻 IFT27 与其脊椎动物同源物一样，不参与鞭毛组装和胞质分裂。摘要：衣藻 IFT25 不参与鞭毛组装，但需要从鞭毛输出 BBSome。
Intraflagellar transport (IFT) particles are composed of polyprotein complexes IFT-A and IFT-B as well as cargo adaptors such as the BBSome. Two IFT-B subunits, IFT25 and IFT27 were found to form a heterodimer, which is essential in exporting the BBSome out of the cilium but not involved in flagellar assembly and cytokinesis in vertebrates. Controversial results were, however, recorded to show that defects in IFT, flagellar assembly and even cytokinesis were caused by IFT27 knockdown in Chlamydomonas reinhardtii. Using C. reinhardtii as a model organism, we report that depletion of IFT25 has no effect on flagellar assembly and does not affect the entry of the BBSome into the flagellum, but IFT25 depletion did impair BBSome movement out of the flagellum, clarifying the evolutionally conserved role of IFT25 in regulating the exit of the BBSome from the flagellum cross species. Interestingly, depletion of IFT25 causes dramatic reduction of IFT27 as expected, which does not cause defects in flagellar assembly and cytokinesis in C. reinhardtii. Our data thus support that Chlamydomonas IFT27, like its vertebrate homologues, is not involved in flagellar assembly and cytokinesis. Summary: Chlamydomonas IFT25 is not involved in flagellar assembly, but is required for export of the BBSome from the flagellum.
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影响因子：
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作者：
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影响因子：
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