Examination of nicotine and saccharin reward in the Goto-Kakizaki diabetic rat model.
Examination of nicotine and saccharin reward in the Goto-Kakizaki diabetic rat model.
复制标题
Goto-Kakizaki 糖尿病大鼠模型中尼古丁和糖精奖励的检查。
DOI：
10.1016/j.neulet.2020.134825
复制
发表时间：
2020
影响因子：
2.5
通讯作者：
Nazarian,Arbi
中科院分区：
文献类型：
--
作者：
Richardson,JanellR;O'Dell,LauraE;Nazarian,Arbi
在美国，2型糖尿病的发病率和死亡率呈指数级增长。吸烟的2型糖尿病患者亚群风险极高，与血糖正常的吸烟者相比，戒烟成功率降低。我们实验室的临床前研究表明，尼古丁的奖励作用在链脲佐菌素和高脂饮食的糖尿病啮齿动物模型中得到增强。目前尚不清楚这种尼古丁奖赏的增强是否可以在其他胰岛素抵抗大鼠模型中得到证实。本研究旨在确定在Goto-Kakizaki（GK）大鼠中是否发现了类似的尼古丁奖励增加，GK大鼠是近交系Wistar大鼠自发形成胰岛素抵抗的模型。本实验观察了SD、Wistar和GK大鼠的尼古丁条件性位置偏爱（CPP）。在SD和Wistar大鼠中发现了稳健的尼古丁CPP，但在GK大鼠中未检测到尼古丁CPP。还在所有三种品系中评价了自发活动，与SD和Wistar大鼠相比，GK大鼠表现出显著更少的活动。为了进一步评估GK大鼠的奖励行为，在48小时内测量糖精溶液的消耗。与SD大鼠相比，GK大鼠糖精摄入量显著增加。这些结果表明，GK大鼠经历了增强的享乐加工相比，SD大鼠。GK大鼠缺乏尼古丁CPP可能是由于学习和记忆缺陷，从而阻碍了它们获得或表达位置偏好的能力。
Morbidity and mortality attributed to type 2 diabetes have exponentially increased in the US. At exceptionally high risk is a subpopulation of persons with type 2 diabetes who smoke, which are shown to have decreased success rates of smoking cessation than euglycemic smokers. Preclinical research in our laboratory has shown that the rewarding effects of nicotine are enhanced in the streptozotocin and high-fat diet rodent model of diabetes. It is presently unclear whether this enhancement of nicotine reward can be demonstrated in other insulin resistant rat models. This study aimed to determine if a similar increase in nicotine reward is found in Goto-Kakizaki (GK) rats, a model of the spontaneous formation of insulin resistance in an inbred sub-strain of Wistar rat. Nicotine conditioned place preference (CPP) was examined in Sprague-Dawley (SD), Wistar, and GK rats. A robust nicotine CPP was found in SD and Wistar rats, but nicotine CPP was not detected in GK rats. Locomotor activity was also evaluated in all three strains, and GK rats demonstrated significantly less activity as compared to SD and Wistar rats. To further assess reward behavior in GK rats, consumption of saccharin solution was measured over a 48 -h period. GK rats showed a significant increase in saccharin intake compared to SD rats. These findings suggest that GK rats experience an enhanced hedonic processing as compared to SD rats. The lack of nicotine CPP in GK rats may be due to deficits in learning and memory, thus hindering their ability to acquire or express a place preference.