Variation of cytokine patterns related to therapeutic response in diffuse cutaneous leishmaniasis
Variation of cytokine patterns related to therapeutic response in diffuse cutaneous leishmaniasis
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DOI：
10.1006/expr.1996.0104
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发表时间：
1996-11-01
影响因子：
2.1
通讯作者：
Barral, A
中科院分区：
文献类型：
--
作者：
Bomfim, G;Nascimento, C;Barral, A
弥漫性皮肤利什曼病是一种罕见疾病，其特征是与利什曼原虫抗原特异性无反应相关的播散性皮肤结节。在弥漫性皮肤利什曼病的活动期，皮肤病变中存在的细胞存在低但并非不存在的 IFN-γ 表达。在这项研究中，我们证实了这一观察结果，并通过显示外周血单核细胞的相似模式以及疾病不同阶段 mRNA 细胞因子表达模式的变化来扩展它。在疾病活动期间，患者不表达 IFN-γ 的 mRNA，而表达 IL-2、IL-4 和 IL-10 的 mRNA。相反，在治疗诱导的皮肤损伤短暂愈合后观察到 IFN-γ 的表达和低 IL-10。在三名患者中，我们能够分析临床复发后获得的第三份 PBMC 样本，记录所有患者的 IFN-γ 表达均降低，但不表达 IL-10。尽管 IFN-γ 表达的出现与临床改善之间存在关联，治疗后 IFN-γ mRNA 显着表达，IL-10 mRNA 表达降低，但这不足以预防这些患者的复发。因此，除了 Th1 和 Th2 平衡的细胞因子特征之外，其他因素可能与弥漫性皮肤利什曼病患者无法产生抗利什曼原虫免疫反应而导致临床改善有关。 (C) 1996 学术出版社
Diffuse cutaneous leishmaniasis is a rare entity characterized by disseminated cutaneous nodules associated with specific anergy to leishmanial antigens. A low but not absent IFN-gamma expression by cells present in cutaneous lesions has been documented during the active phase of diffuse cutaneous leishmaniasis. In this study we confirm this observation, and extend it by showing a similar pattern in peripheral blood mononuclear cells and the variation of mRNA cytokine expression pattern during different stages of the disease. During active disease, patients did not express mRNA for IFN-gamma, while expressing mRNA for IL-2, IL-4, and IL-10. In contrast, an expression of IFN-gamma and low IL-10 was observed after treatment-induced transient healing of cutaneous lesions. In three patients we have been able to analyze a third PBMC sample obtained after clinical relapse, documenting in all of them decreased IFN-gamma expression with no expression of IL-10. Although there was an association between the appearance of IFN-gamma expression and clinical improvement, with marked expression of IFN-gamma mRNA and decreased expression of mRNA for IL-10 after treatment, this was not sufficient to prevent relapse in these patients. Therefore, it is possible that factors other than the cytokines characteristic of the Th1 and Th2 balance are implicated in the inability of diffuse cutaneous leishmaniasis patients to mount an anti-leishmania immune response causing clinical improvement. (C) 1996 Academic Press, Inc.