Sensory rhodopsin II: functional insights from structure.
Sensory rhodopsin II: functional insights from structure.
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感觉视紫红质 II：从结构中了解功能。
DOI：
10.1016/s0959-440x(02)00359-7
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发表时间：
2002
影响因子：
6.8
通讯作者：
Luecke,Hartmut
中科院分区：
文献类型：
--
作者：
Spudich,JohnL;Luecke,Hartmut
盐古菌（广泛存在的微生物视紫红质家族的第一个感觉成员）中感觉视紫红质趋光性受体的原子分辨率结构已经深入了解其膜嵌入传感器的相互作用面以及光谱调谐机制。感觉视紫红质和光驱动质子泵细菌视紫红质之间的光谱差异很大程度上取决于发色团结合袋中保守精氨酸残基的重新定位。从结构中获得的信息，结合生物物理和生化分析，建立了受体激活和信号中继的模型，其中受体中光诱导的螺旋倾斜通过横向跨膜螺旋-螺旋相互作用传递到传感器。
Atomic resolution structures of a sensory rhodopsin phototaxis receptor in haloarchaea (the first sensory member of the widespread microbial rhodopsin family) have yielded insights into the interaction face with its membrane-embedded transducer and into the mechanism of spectral tuning. Spectral differences between sensory rhodopsin and the light-driven proton pump bacteriorhodopsin depend largely upon the repositioning of a conserved arginine residue in the chromophore-binding pocket. Information derived from the structures, combined with biophysical and biochemical analysis, has established a model for receptor activation and signal relay, in which light-induced helix tilting in the receptor is transmitted to the transducer by lateral transmembrane helix–helix interactions.