A convenient in vivo cap donor delivery system to investigate the cap snatching of plant bunyaviruses
A convenient in vivo cap donor delivery system to investigate the cap snatching of plant bunyaviruses
复制标题
一种方便的体内帽供体递送系统，用于研究植物布尼亚病毒的帽抢夺
DOI：
10.1016/j.virol.2019.10.017
复制
发表时间：
2020-01-02
期刊：
影响因子：
3.7
通讯作者：
Du, Zhenguo
中科院分区：
文献类型：
--
作者：
Lin, Wenzhong;Wu, Ran;Du, Zhenguo
与感染动物的同类病毒一样，植物布尼亚病毒使用从宿主细胞mRNAs上切割出来的带帽RNA前导来启动病毒基因组转录，这一过程被称为帽子抢夺，但它们缺乏体内系统来研究这一过程的细节。在这里，我们报道了水稻条纹细小病毒(RSV)和番茄斑点萎萎病病毒(TSWV)从农业渗透瞬时表达的mRNAs中切割帽RNA前导，这使得人工将定义的帽供体转移到这两种植物布尼亚病毒成为可能，具有前所未有的便利。有了这个系统，关于植物布尼亚病毒如何选择和使用带帽的RNA前导的一些想法可以很容易地得到测试。我们还能够获得明确的证据，即TSWV选择的封顶RNA领导通常比RSV选择的要长。TSWV经常在转录中使用启动和重排机制，启动时的封顶RNA前导长度小于一定长度，就像最近对RSV所证明的那样。
Like their animal-infecting counterparts, plant bunyaviruses use capped RNA leaders cleaved from host cellular mRNAs to prime viral genome transcription in a process called cap-snatching, but in vivo systems to investigate the details of this process are lacking for them. Here, we report that Rice stripe tenuivirus (RSV) and Tomato spotted wilt tospovirus (TSWV) cleave capped RNA leaders from mRNAs transiently expressed by agroinfiltration, which makes it possible to artificially deliver defined cap donors to the two plant bunyaviruses with unprecedented convenience. With this system, some ideas regarding how plant bunyaviruses select and use capped RNA leaders can be tested easily. We were also able to obtain clear evidence that the capped RNA leaders selected by TSWV are generally longer than those by RSV. TSWV frequently uses the prime-and-realign mechanism in transcription primed by capped RNA leaders shorter than a certain length, like that has been demonstrated recently for RSV.