Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis
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DOI：
10.1073/pnas.0611629104
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发表时间：
2007-04-10
影响因子：
11.1
通讯作者：
Saito, Kazuki
中科院分区：
文献类型：
--
作者：
Hirai, Masami Yokota;Sugiyama, Kenjiro;Saito, Kazuki
了解植物代谢作为一个综合系统是必不可少的代谢工程，旨在有效地生产对人类生活和全球环境有用的化合物。“组学”方法将转录组和代谢组数据整合到单个数据集中，并且可以导致识别参与感兴趣的代谢途径的未知基因及其调控网络。植物中一种有趣但描述不多的代谢途径是芥子油苷（GSL）的生物合成，芥子油苷（GSL）是一组衍生自在拟南芥科中发现的氨基酸的生物活性次级产物。在这里，我们报告了两个R2 R3-Myb转录因子的发现，积极控制GSL的生物合成在拟南芥的集成组学方法。公开可用的、条件无关的数据和条件特异性的（即，硫缺乏）数据鉴定了Myb 28和Myb 29作为特异性参与调节脂肪族GSL产生的候选转录因子基因。基因敲除突变体和异位表达的分析表明，Myb 28是一个积极的调节基础水平生产的脂肪GSL。Myb 29可能在茉莉酸甲酯介导的一组脂肪族GSL生物合成基因的诱导中起辅助作用。Myb 28在正常情况下不合成GSL的拟南芥培养的悬浮细胞中的过表达导致了大量GSL的产生，这表明通过操纵这些转录因子来高效工业生产GSL的可能性。一个工作模式，涉及这些基因的GSL生产调节，生产蛋氨酸衍生的硫代葡萄糖苷（PMG）1和2，被假定。
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. one of the intriguing, although poorly described metabolic pathways in plants is the biosynthesis of glucosinolates (GSLs), a group of bioactive secondary products derived from amino acids that are found in the family Brassicaceae. Here we report the discovery of two R2R3-Myb transcription factors that positively control the biosynthesis of GSLs in Arabidopsis thaliana by an integrated omics approach. Combined transcriptome coexpression analysis of publicly available, condition-independent data and the condition-specific (i.e., sulfur-deficiency) data identified Myb28 and Myb29 as candidate transcription factor genes specifically involved in the regulation of aliphatic GSL production. Analysis of a knockout mutant and ectopic expression of the gene demonstrated that Myb28 is a positive regulator for basal-level production of aliphatic GSLs. Myb29 presumably plays an accessory function for methyl jasmonate-mediated induction of a set of aliphatic GSL biosynthetic genes. Overexpression of Myb28 in Arabidopsis-cultured suspension cells, which do not normally synthesize GSLs, resulted in the production of large amounts of GSLs, suggesting the possibility of efficient industrial production of GSLs by manipulation of these transcription factors. A working model for regulation of GSL production involving these genes, renamed Production of Methionine-Derived Glucosinolate (PMG) 1 and 2, are postulated.