EFFECTS OF LIPID ENVIRONMENT ON THE LIGHT-INDUCED CONFORMATIONAL-CHANGES OF RHODOPSIN .2. ROLES OF LIPID CHAIN-LENGTH, UNSATURATION, AND PHASE STATE
EFFECTS OF LIPID ENVIRONMENT ON THE LIGHT-INDUCED CONFORMATIONAL-CHANGES OF RHODOPSIN .2. ROLES OF LIPID CHAIN-LENGTH, UNSATURATION, AND PHASE STATE
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DOI：
10.1021/bi00332a007
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
HUBBELL, WL
中科院分区：
文献类型：
--
作者：
BALDWIN, PA;HUBBELL, WL
当视紫红质被结合到饱和的短链磷脂二肉豆蔻基磷脂酰胆碱中时，蛋白质的光解导致了一系列异常的光谱转换，而后视紫红质I衰变的主要产物是游离的视网膜加视蛋白[Baldwin，P.A.，and Hubbell，W.L.(1985)]。通过将视紫红质掺入一系列特定组成的磷脂酰胆碱中，确定了导致光谱行为改变的脂环境的性质。在含有太短的酰基链的双层膜中没有发现显着数量的变紫红质II(。14个碳原子长)，在只有正构烷基链存在的情况下，或低于重组膜的特征相变温度。后视紫红质II中间体的形成不需要双键，正如在二植酰磷脂酰胆碱重组体中观察到的那样。
When rhodopsin is incorporated into the saturated short-chain phospholipid dimyristoylphosphatidylcholine, photolysis of the protein results in an abnormal sequence of spectral transitions, and the dominant product of metarhodopsin I decay is free retinal plus opsin [Baldwin, P.A., and Hubbell, W. L. (1985)]. By incorporation of rhodopsin into a series of phosphatidylcholines of defined composition, the properties of the lipid environment that are responsible for the altered spectral behavior were determined. Metarhodopsin II is not found in appreciable amounts in bilayers containing acyl chains that are too short (.ltoreq. 14 carbon atoms in length), in the presence of only n-alkyl chains, or below the characteristic phase-transition temperature of recombinant membranes. Double bonds are not required for the formation of the metarhodopsin II intermediate, as it is observed in diphytanoylphosphatidylcholine recombinants.