Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia
Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia
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DOI：
10.1128/aac.01688-08
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发表时间：
2009-08-01
影响因子：
4.9
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
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作者：
Wakabayashi, Hiroyuki;Yamauchi, Koji;Yoshie, Hiromasa
乳铁蛋白（LF）是一种存在于唾液和龈沟液中的铁结合抗菌蛋白，它可能与宿主防御口腔病原体（包括牙周病细菌）有关。在本研究中，我们评估了LF相关剂对两种牙周病细菌牙龈卟啉单胞菌和中间普雷沃菌的生长和生物膜形成的体外影响，这两种细菌作为生物膜存在于龈下菌斑中。通过与≥ 130 μ g/ml的人LF（hLF）、无铁和铁饱和的牛LF（分别为apo-bLF和holo-bLF）以及≥ 6 μ g/ml的bLF衍生的抗菌肽乳铁蛋白B（LFcin B）孵育，牙龈卟啉单胞菌和中间卟啉单胞菌的生长被抑制长达5小时;但这些作用在8小时后很弱。较低浓度（>= 8 μ g/ml）的bLF和hLF的各种铁结合形式（apo、天然和holo形式）而不是LFcin B有效地抑制了牙龈卟啉单胞菌和中间卟啉单胞菌在24小时内的生物膜形成。牙龈卟啉单胞菌和中间卟啉单胞菌的预形成的生物膜也通过与各种铁结合的bLF、hLF和LFcin B孵育5小时而减少。在天然bLF与四种抗生素组合使用时的有效性的检查中，发现与单独使用每种药剂实现的减少水平相比，用环丙沙星、克拉霉素和米诺环素与天然bLF组合处理24小时减少牙龈卟啉单胞菌的预形成生物膜的量。这些结果表明LF对牙龈卟啉单胞菌和中间卟啉单胞菌具有较低的铁依赖性的牙周膜活性，以及LF用于预防和治疗牙周疾病以及作为牙周疾病的辅助治疗的潜在有用性。
Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >= 130 mu g/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >= 6 mu g/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (>= 8 mu g/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.