Follicle formation in the canine ovary after autografting to a peripheral site.
Follicle formation in the canine ovary after autografting to a peripheral site.
复制标题
自体移植到周围部位后犬卵巢中卵泡的形成。
DOI：
10.1111/j.1439-0531.2011.01880.x
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. Taniguchi and T. Otoi
中科院分区：
文献类型：
--
作者：
T. Terazono;Y. Kaedei;F. Tanihara;Z. Namura;V. L. Viet;M. Takagi;M. Inoue;Y. Sato;M. Taniguchi and T. Otoi
内容本研究报告了犬卵巢组织在胸腰肌筋膜下自体移植后表面上的卵泡发育以及卵泡衍生的卵母细胞在成熟培养后减数分裂的恢复。从母犬身上切除卵巢后，将每对卵巢切成大约一半。将半卵巢移植到母犬的三个不同身体部位（股四头肌和胸腰肌筋膜下，以及肩胛区的三角肌中）。在移植后35天从母犬中回收所有移植的卵巢。在胸腰筋膜下移植的卵巢表面可见窦状卵泡。组织学检查显示，在不同的发展阶段，无论移植部位的活卵泡。不同发育阶段卵泡颗粒细胞大多表达增殖细胞核抗原（PCNA）。从移植在胸腰肌筋膜下的卵巢中收集总共三个卵母细胞，其中卵母细胞在成熟培养后达到中期I。这是第一份报告，以证明卵泡发育和减数分裂恢复的卵母细胞回收自体移植犬卵巢组织。
ContentsThis study reports about follicular development on the surface of canine ovarian tissue after autografting under the fascia of the thoracolumbar muscle and about meiotic resumption of follicle‐derived oocyte after maturation culture. After ovarian excision from a bitch, each ovary of the pairs was cut approximately into half. The hemi‐ovaries were transplanted into the bitch of origin at three different body sites (under the fascia of the quadriceps femoris muscle and the thoracolumbar muscle, and in the deltoid muscle in the scapular region). All grafted ovaries were recovered from the bitch at 35 days post‐transplantation. A visible antral follicle was observed on the surface of the ovary grafted under the thoracolumbar fascia. Histological examination revealed viable follicles at different stages of development irrespective of graft site. Most granulosa cells in the follicles at different stages of development expressed proliferating cell nuclear antigen (PCNA). A total of three oocytes were collected from an ovary grafted under the fascia of the thoracolumbar muscle, wherein an oocyte reached metaphase I after maturation culture. This is the first report to demonstrate follicular development and meiotic resumption of oocytes recovered from autografted canine ovarian tissues.