QUANTITATION OF RELATIVE FITNESS AND GREAT ADAPTABILITY OF CLONAL POPULATIONS OF RNA VIRUSES
QUANTITATION OF RELATIVE FITNESS AND GREAT ADAPTABILITY OF CLONAL POPULATIONS OF RNA VIRUSES
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DOI：
10.1128/jvi.65.6.2960-2967.1991
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发表时间：
1991-06-01
影响因子：
5.4
通讯作者：
DUARTE, E
中科院分区：
文献类型：
--
作者：
HOLLAND, JJ;DELATORRE, JC;DUARTE, E
我们描述了一个敏感的，内部控制的方法比较恒定或变化的宿主环境中的病毒种群的遗传适应性和相对适合度。水泡性口炎病毒的某些单克隆抗体抗性突变体在连续传代期间可以与它们所来源的亲本野生型克隆在混合物中平等竞争。这些遗传标记的“替代野生型”中性突变体，当与野生型病毒混合时，允许可靠地测量病毒适应性和病毒适应不同宿主环境的变化。定量适应度矢量图以图形方式表明，即使是RNA病毒的克隆也由复杂的变异群体（准种）组成。在新宿主细胞或动物中，在病毒克隆的极少数传代中选择适应性更强（竞争性复制能力）的变体。即使是很好地适应BHK 21细胞的克隆，在BHK 21细胞中重复传代期间也获得了进一步的适应性。
We describe a sensitive, internally controlled method for comparing the genetic adaptability and relative fitness of virus populations in constant or changing host environments. Certain monoclonal antibody-resistant mutants of vesicular stomatitis virus can compete equally during serial passages in mixtures with the parental wild-type clone from which they were derived. These genetically marked "surrogate wild-type" neutral mutants, when mixed with wild-type virus, allow reliable measurement of changes in virus fitness and of virus adaptation to different host environments. Quantitative fitness vector plots demonstrate graphically that even clones of an RNA virus are composed of complex variant populations (quasispecies). Variants of greater fitness (competitive replication ability) were selected within very few passages of virus clones in new host cells or animals. Even clones which were well adapted to BHK21 cells gained further fitness during repeated passages in BHK21 cells.