Effects of blue light on pigment biosynthesis of Monascus
Effects of blue light on pigment biosynthesis of Monascus
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DOI：
10.1007/s12275-016-6011-1
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发表时间：
2016-04-01
影响因子：
3
通讯作者：
Wang, Changlu
中科院分区：
文献类型：
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作者：
Chen, Di;Xue, Chunmao;Wang, Changlu
研究了不同蓝光照射水平对红曲霉M9菌株生长和细胞内色素产量的影响。与黑暗相比，持续暴露在100勒克斯蓝光下会降低六种色素的产量，即红斑红胺（RUM）、红斑红胺（MOM）、红斑素（RUN）、红斑红蛋白（MON）、红红素（MS）和红黄素（AK）。然而，暴露于不同水平的蓝光对色素产生有不同的影响。与未暴露相比，每天暴露在 100 勒克斯蓝光下一次 30 分钟，以及每天暴露在 100 勒克斯蓝光下一次和两次 15 分钟，可以增强 RUM、MOM、MS 和 AK 的产生，并减少 RUN 和 MON。暴露于 100 勒克斯的光照下两次，每次 30 分钟/天，以及暴露于 200 勒克斯的光照下一次，每次 45 分钟/天，会降低 RUM、MOM、MS 和 AK 产量，并增加 RUN 和 MON。同时，通过实时定量PCR分析色素生物合成基因的表达水平。结果表明，基因MpPKS5、mppR1、mppA、mppB、mmpC、mppD、MpFasA、MpFasB和mppF与RUN和MON的产量呈正相关，而mppE和mppR2与RUM、MOM、MS和AK的产量相关。
The influence of different illumination levels of blue light on the growth and intracellular pigment yields of Monascus strain M9 was investigated. Compared with darkness, constant exposure to blue light of 100 lux reduced the yields of six pigments, namely, rubropunctatamine (RUM), monascorubramine (MOM), rubropunctatin (RUN), monascorubrin (MON), monascin (MS), and ankaflavin (AK). However, exposure to varying levels of blue light had different effects on pigment production. Exposure to 100 lux of blue light once for 30 min/day and to 100 lux of blue light once and twice for 15 min/day could enhance RUM, MOM, MS, and AK production and reduce RUN and MON compared with non-exposure. Exposure to 100 lux twice for 30 min/day and to 200 lux once for 45 min/day decreased the RUM, MOM, MS, and AK yields and increased the RUN and MON. Meanwhile, the expression levels of pigment biosynthetic genes were analyzed by real-time quantitative PCR. Results indicated that gene MpPKS5, mppR1, mppA, mppB, mmpC, mppD, MpFasA, MpFasB, and mppF were positively correlated with the yields of RUN and MON, whereas mppE and mppR2 were associated with RUM, MOM, MS, and AK production.