Corneal transplantation with donor tissue kept in organ culture for 7 weeks
Corneal transplantation with donor tissue kept in organ culture for 7 weeks
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DOI：
10.1034/j.1600-0420.1999.770306.x
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发表时间：
1999-06-01
期刊：
影响因子：
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通讯作者：
Hjortdal, JO
中科院分区：
文献类型：
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作者：
Ehlers, H;Ehlers, N;Hjortdal, JO
目的：方法：对6例对称性眼病患者行双侧角膜移植术，其中1眼移植常规培养角膜（平均16 d），另1眼移植供体角膜（平均49 d）。结果进行了评估，生物显微镜，移植物厚度，内皮细胞密度和视觉性能后，观察时间至少为1 year.Results：穿透7-8 mm移植是在所有情况下并不复杂。两组的内皮细胞密度范围为1000-2000个细胞/mm（2），无其他眼部病变的病例的视力为0.2-0.9。术后移植物厚度和去肿胀之间没有显着差异2和7周培养的角膜，在最后检查的厚度分别为0.50毫米和0.49毫米2和7周culturescorneas.Conclusion：7周培养的角膜的临床结果相比，使用较短的培养周期。延长培养期可用于改善移植物的其他质量（相容性、细胞数量、细胞代谢）和微生物控制。
Purpose: To study the fate of corneal grafts after extended organ culture (7 weeks).Methods: Six patients with symmetrical eye diseases were grafted bilaterally, in one eye with a cornea prepared by routine organ culture (mean 16 days), in the other eye with a donor cornea kept for 7 weeks (mean 49 days) in organ culture. The outcome was evaluated by biomicroscopy, graft thickness, endothelial cell density and visual performance after an observation time of at least 1 year.Results: Penetrating 7-8 mm grafting was uncomplicated in all cases. The endothelial densities were in both groups in the range 1000-2000 cells/mm(2), and visual acuity 0.2-0.9 in cases with no other ocular pathology. Postoperative graft thickness and deswelling did not differ between 2- and 7-week cultured corneas, At final examination the thicknesses were 0.50 mm and 0.49 mm for 2- and 7-weeks cultured corneas.Conclusion: Seven-week cultured corneas give clinical results comparable to those obtained using shorter culture periods. An extended culture period may be used to improve other qualities of the graft (compatibility, cell number, cell metabolism) and microbiological control.