Uncovering the Design Rules for Peptide Synthesis of Metal Nanoparticles
Uncovering the Design Rules for Peptide Synthesis of Metal Nanoparticles
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DOI：
10.1021/ja907454f
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发表时间：
2010-04-28
影响因子：
15
通讯作者：
Wang, Daniel I. C.
中科院分区：
文献类型：
--
作者：
Tan, Yen Nee;Lee, Jim Yang;Wang, Daniel I. C.
肽是多功能试剂（还原剂和封端剂），可用于在相对温和的条件下合成生物相容性金属纳米颗粒。然而，由于缺乏肽设计规则，金属纳米颗粒的肽合成进展缓慢。很难从分离自生物来源的肽（例如，生物矿化生物）或通过组合展示文库选择，因为它们的组成和结构变化很大。肽段中大量的随机和非活性氨基酸序列也增加了知识提取的难度。在这项研究中，一个“自下而上”的方法是用来制定一套基本规则的大小和形状控制的肽合成的金纳米粒子的性质的20个天然α-氨基酸AuCl 4-还原和结合Au-0。发现肽的还原能力取决于某些还原性氨基酸残基的存在，其活性可能受到具有不同Au-0结合强度的邻近残基的调节。另一个发现是肽净电荷对Au纳米颗粒的成核和生长的影响。在这些理解的基础上，设计了几种多功能肽来合成不同形态（纳米球和纳米片）的金纳米颗粒，并且通过在肽序列中选择氨基酸残基的战略位置来调节尺寸。这里提出的方法和研究结果是有用的，为合理设计用于合成金属纳米结构的肽建立科学基础。
Peptides are multifunctional reagents (reducing and capping agents) that can be used for the synthesis of biocompatible metal nanoparticles under relatively mild conditions. However, the progress in peptide synthesis of metal nanoparticles has been slow due to the lack of peptide design rules. It is difficult to establish sequence-reactivity relationships from peptides isolated from biological sources (e.g., biomineralizing organisms) or selected by combinatorial display libraries because of their widely varying compositions and structures. The abundance of random and inactive amino acid sequences in the peptides also increases the difficulty in knowledge extraction. In this study, a "bottom-up" approach was used to formulate a set of rudimentary rules for the size- and shape-controlled peptide synthesis of gold nanoparticles from the properties of the 20 natural alpha-amino acids for AuCl4- reduction and binding to Au-0. It was discovered that the reduction capability of a peptide depends on the presence of certain reducing amino acid residues, whose activity may be regulated by neighboring residues with different Au-0 binding strengths. Another finding is the effect of peptide net charge on the nucleation and growth of the Au nanoparticles. On the basis of these understandings, several multifunctional peptides were designed to synthesize gold nanoparticles in different morphologies (nanospheres and nanoplates) and with sizes tunable by the strategic placement of selected amino acid residues in the peptide sequence. The methodology presented here and the findings are useful for establishing the scientific basis for the rational design of peptides for the synthesis of metal nanostructures.