Heme-a, the heme prosthetic group of cytochrome c oxidase, is increased in Alzheimer's disease.
Heme-a, the heme prosthetic group of cytochrome c oxidase, is increased in Alzheimer's disease.
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DOI：
10.1016/j.neulet.2009.06.007
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发表时间：
2009-09-25
影响因子：
2.5
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Dwyer BE;Stone ML;Gorman N;Sinclair PR;Perry G;Smith MA;Zhu X
血红素-a 是细胞色素 C 氧化酶 (COX) 的血红素辅基，是线粒体电子传递链的末端复合物。我们测量了 9 名被诊断为痴呆症的患者死后脑组织中的血红素 A 水平：5 名患者的主要诊断为阿尔茨海默病 (AD)，2 名患者诊断为 AD/弥漫性路易体病 (DLBD)，1 名患者诊断为 DLBD，1 名患者诊断为 DLBD（重度）/AD（轻度）。八名死于非神经系统原因的非痴呆患者作为对照。当初步诊断为 AD（AD 和 AD/DLBD）时，与对照组相比，以蛋白质计算的脑血红素 a 水平几乎增加两倍 (p<0.001)。使用灌注和未灌注的大鼠，我们发现测量的脑血红素-a 水平不受脑组织中血液存在的影响。在小鼠中，我们发现冷冻前在 4°C 下储存 24 小时不会影响脑血红素-a 的水平。这些动物研究表明，AD 中脑血红素 a 水平的增加并不是由于死后大脑中的血液或死后间隔的变化所致。
Heme-a, is the heme prosthetic group of cytochrome c oxidase (COX), the terminal complex of the mitochondrial electron transport chain. We measured heme-a levels in postmortem brain tissue from nine patients diagnosed with dementia: Alzheimer’s disease (AD) was the primary diagnosis in five, AD/Diffuse Lewy body disease (DLBD) was diagnosed in two, DLBD was diagnosed in one, and DLBD (severe)/AD (mild) was diagnosed in one. Eight non-demented patients who died from non-neurological causes served as controls. When the primary diagnosis was AD (AD and AD/DLBD), levels of cerebral heme-a were increased almost two-fold on a protein basis compared to controls (p<0.001). Using perfused and unperfused rats we showed that measured levels of cerebral heme-a were unaffected by the presence of blood in brain tissue. In mice we showed that levels of cerebral heme-a were unaffected by 24 hours of storage at 4°C prior to freezing. These animal studies suggest that increased levels of cerebral heme-a in AD were not due to blood in postmortem brain or variation in postmortem interval.
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