BOTH SUBUNITS OF RAT-LIVER FERRITIN ARE REGULATED AT A TRANSLATIONAL LEVEL BY IRON INDUCTION
BOTH SUBUNITS OF RAT-LIVER FERRITIN ARE REGULATED AT A TRANSLATIONAL LEVEL BY IRON INDUCTION
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DOI：
10.1093/nar/14.2.915
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发表时间：
1986-01-24
影响因子：
14.9
通讯作者：
MUNRO, HN
中科院分区：
文献类型：
--
作者：
AZIZ, N;MUNRO, HN
给大鼠施用铁后几小时，肝脏铁蛋白合成增加数倍。然而，用cDNA探针对铁蛋白轻（L）和重（H）亚基mRNA进行的北方印迹分析未能显示任一信使的总群体增加。因此，在对照组和在3.5小时处死的铁给药大鼠中研究了铁蛋白信息的细胞质分布。在蔗糖梯度上分级分离肝脏线粒体后上清液以分离多核糖体、单体、核糖体亚基和细胞汁液。从每个组分中提取RNA并使用北方印迹法分析，结果表明，每个亚基的总mRNA群体的65%存在于对照大鼠的细胞汁液中，可能是作为mRNP颗粒，因为该组分中不存在核糖体RNA。铁给药后，这些游离mRNA储备被募集到多核糖体上，使游离mRNA库减少到总量的15%。我们认为这是由于铁进入细胞时激活了受阻的铁蛋白信息。
A few hours after administering iron to rats, liver ferritin synthesis increases several fold. However, Northern blot analysis with cDNA probes for ferritin light (L) and heavy (H) subunlt mRNAs failed to show an increase in total population of either messenger. Cytoplasmic distribution of ferritin messages was therefore investigated in control and iron administered rats killed at 3.5 hours. The liver post-mitochondrial supernatant was fractionated on a sucrose gradient to separate polyribosomea, monosomes, ribosomal subunits and cell sap. RNA extracted from each fraction and analyzed using Northern blotting showed that 65% of the total mRNA population for each subunit was present in the cell sap of control rats, presumably as mRNP particles since ribosomal RNA was absent from this fraction. After iron administration, these reserves of free mRNA were recruited onto the polysomes, reducing the free mRNA pool to 15% of the total. We interpret this to be due to activation of blocked ferritin messages on entry of iron Into the cell.