3D-printed surface promoting osteogenic differentiation and angiogenetic factor expression of BMSCs on Ti6Al4V implants and early osseointegration in vivo
3D-printed surface promoting osteogenic differentiation and angiogenetic factor expression of BMSCs on Ti6Al4V implants and early osseointegration in vivo
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3D打印表面促进Ti6Al4V种植体上BMSC的成骨分化和血管生成因子表达以及体内早期骨整合
DOI：
10.1016/j.jmst.2018.09.063
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发表时间：
2019-02-01
影响因子：
10.9
通讯作者：
Chen, Fengshan
中科院分区：
文献类型：
--
作者：
Zhang, Jinkai;Zhou, Wenhui;Chen, Fengshan
三维打印（3D-P）钛植入物显示出许多优点，例如设计灵活性、更高的效率、容易构建复杂或定制结构的能力等，并被认为有可能取代传统的植入物。然而，3D-P钛表面的生物学性能尚未得到系统的研究。本文分析了3D-P钛合金表面的特性，并在体外观察了骨髓间充质干细胞（BMSCs）对3D-P表面的生物学反应。植入大鼠股骨髁3周和6周后，评价骨整合性能。结果表明，3D-P Ti6 Al 4V种植体具有明显的宏观起伏粗糙表面和相对较好的亲水性，促进了BMSCs的粘附、增殖、成骨分化和血管生成因子的表达。此外，在体内骨整合性能也优于对照组在早期阶段。本研究表明，3D-P钛合金是一种很有前途的候选材料，用作植入物。(C)2018由Elsevier Ltd代表Journal of Materials Science & Technology编辑部出版。
Three-dimensional-printed (3D-P) titanium implants display many advantages, such as design flexibility, higher efficiency, the capability to easily construct complex or customized structures, etc., and is believed to potentially replace traditional implants. However, the biological performance of the 3D-P titanium surface has not been investigated systematically. Herein, we analyzed the surface characteristics of 3D-P Ti6Al4V implants and evaluated the biological responses of bone marrow derived mesenchymal stromal cells (BMSCs) to the 3D-P surface in vitro. Moreover, after implantation into the rat femoral condyle for 3 and 6 weeks, the osseointegration performance was evaluated. The results showed the 3D-P Ti6Al4V implant presented distinct fluctuant macroscale rough surface and relatively better hydrophilicity which enhanced the adhesion, proliferation, osteogenic differentiation and angiogenetic factor expression of BMSCs. Moreover, the in vivo osseointegration performance was also better than that of the control group at the early stage. The present study suggested the 3D-P titanium alloy is a promising candidate to be used as implant material. (C) 2018 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.