From the genome to the proteome-biomarkers in colorectal cancer
From the genome to the proteome-biomarkers in colorectal cancer
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DOI：
10.1007/s00423-007-0230-1
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发表时间：
2008-01-01
影响因子：
2.3
通讯作者：
Roblick, Uwe J.
中科院分区：
文献类型：
--
作者：
Habermann, Jens K.;Bader, Franz G.;Roblick, Uwe J.
背景和目的结直肠癌是癌症相关死亡的第二大原因。目前结肠直肠癌筛查的临床实践（粪便隐血试验，FOBT；结肠镜检查）有助于降低死亡率。然而，尽管有这些筛查项目，约70%的癌症在肿瘤晚期（UICC III/IV）被发现，患者预后较差。因此，用于早期癌症检测的创新工具和方法可以直接提高患者的存活率。近年来，随着全球基因和蛋白质表达谱等技术的出现，生物医学研究进展迅速。综合肿瘤谱分析已成为一个深入研究的领域，旨在识别与改进诊断和治疗相关的生物标志物。在本文中，我们报道了基因组学、转录组学和蛋白质组学方法在组织和血液中的生物标志物鉴定的全面综述，主要强调了二维凝胶电泳（2-DE）和质谱分析。结论基于蛋白质组学的诊断技术具有较高的敏感性和特异性，可用于健康患者和肿瘤患者的鉴别，并可大大改进常用的分类系统和诊断方法。然而，这一进展尚未从实验室转移到临床，但可能为更准确、更有针对性的个性化药物打开大门，从而提高患者的生存率。
Background and aims Colorectal cancer is the second leading cause of cancer-related death. Current clinical practice in colorectal cancer screening (fecal occult blood test, FOBT; colonoscopy) has contributed to a reduction of mortality. However, despite these screening programs, about 70% of carcinomas are detected at advanced tumor stages (UICC III/IV) presenting poor patient prognosis. Thus, innovative tools and methodologies for early cancer detection can directly result in improving patient survival rates.Patients/methods Biomedical research has advanced rapidly in recent years with the availability of technologies such as global gene and protein expression profiling. Comprehensive tumor profiling has become a field of intensive research aiming at identifying biomarkers relevant for improved diagnostics and therapeutics.Results In this paper, we report a comprehensive review of genomic, transcriptomic, and proteomic approaches for biomarker identification in tissue and blood with a main emphasis on two-dimensional gel-electrophoresis (2-DE) and mass spectrometry analyses.Conclusion Proteomics-based technologies enable to distinguish the healthy patient from the tumor patient with high sensitivity and specificity and could greatly improve common classification systems and diagnostics. However, this progress has not yet been transferred from bench to bedside but could open the door to a more accurate and target specific personalized medicine with improved patient survival.