Rabbit retinal ganglion cell survival after optic nerve section and its effect on the inner plexiform layer
Rabbit retinal ganglion cell survival after optic nerve section and its effect on the inner plexiform layer
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DOI：
10.1016/j.exer.2003.09.012
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
de la Villa, P
中科院分区：
文献类型：
--
作者：
Germain, F;Calvo, M;de la Villa, P
本文观察了兔视神经切断后视网膜内层的结构变化。视神经内注射固蓝标记视网膜神经节细胞（Retinal ganglion cells，RGC），在荧光灯下计数对照组和切断视神经后7、14、21和26天视网膜的RGC。还进行了视网膜横截面的研究。为此，视网膜切片用苏木精-伊红染色，并用免疫组织化学法检测GABA（A）受体的α 1和β 2/β 3亚单位。（视纹中1086 +/- 173个细胞mm（-2），外周中119 +/- 46个细胞mm（-2），平均值+/- SD，n = 5）。在轴突切断后14天，271 +/- 46个细胞mm（-2）保留在视纹中，33 +/- 6个细胞mm（-2）保留在外周中，对应于27%的平均存活率。神经节细胞数量在随后的日子里进一步减少，在ONS后1个月达到7.54%。在视网膜横截面中也观察到内丛状层和神经节细胞层的厚度显著减少。免疫细胞化学研究表明，一个显着的紊乱层分层的内丛状层（IPL）。我们的结论是，ONS后，RGC死亡主要发生在7和14天之间的轴突切断术后和进行性死亡长达26天，导致IPL的厚度和随后的层的解体的减少。(C)2004爱思唯尔有限公司保留所有权利。
Structural modifications of the inner retina were studied after optic nerve section (ONS) in the rabbit. Retinal ganglion cells (RGC) were labelled by injection of Fast Blue into the optic nerve, and counted under fluorescent light in control retina and retina 7, 14, 21 and 26 days post-axotomy. Studies on retinal cross-sections were also performed. For this purpose, retinal sections were stained with haematoxylin-eosin and immunohystochemistry for alpha1 and beta2/beta3 sub-units of the GABA(A) receptors.One week after axotomy, there was no significant loss in the number of ganglion cells with respect to control counts (1086 +/- 173 cells mm(-2) in the visual streak and 119 +/- 46 cells mm(-2) in the periphery, mean +/- SD, n = 5). At 14 days post-axotomy, 271 +/- 46 cells mm(-2) remained in the visual streak and 33 +/- 6 cells mm(-2) in the periphery, corresponding to a mean survival of 27%. The number of ganglion cells decreased further on the following days, reaching 7.54% 1 month after ONS. A significant reduction in the thickness of the inner plexiform and ganglion cell layers was also observed in retinal cross-sections. Immunocytochemical studies show a remarkable disorganization of the layer stratification in the inner plexiform layer (IPL). We conclude that after ONS, RGC death occurs mainly between 7 and 14 days post-axotomy and a progressive death up to 26 days, causing a decrease in the thickness of the IPL and subsequent disorganization of its layers. (C) 2004 Elsevier Ltd. All rights reserved.