Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort.
Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort.
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DOI：
10.1186/s40168-016-0213-y
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发表时间：
2017-01-17
期刊：
影响因子：
15.5
通讯作者：
Stanton C
中科院分区：
文献类型：
--
作者：
Hill CJ;Lynch DB;Murphy K;Ulaszewska M;Jeffery IB;O'Shea CA;Watkins C;Dempsey E;Mattivi F;Tuohy K;Ross RP;Ryan CA;O' Toole PW;Stanton C
肠道是人类微生物组中研究最广泛的生态位。本研究的目的是描述一组 1 至 24 周龄母乳喂养婴儿 (n = 192) 的初始肠道微生物群发育特征。 V4-V5 区 16S rRNA 扩增子 Illumina 测序，同时进行细菌学培养。还通过 LC-MS 检查了 4 周龄婴儿尿液的代谢组学特征。在 24 周的检查期间，足月 (FT)、自然阴道分娩 (SVD) 婴儿的微生物群在门和属水平上保持稳定。与 FT-SVD 婴儿相比，FT 剖腹产 (CS) 婴儿出生第一周后粪便中厚壁菌门丰度增加 (p<0.01)，放线菌丰度较低 (p<0.001)。 FT-CS 婴儿在出生后第 8 周逐渐发展出与 FT-SVD 非常相似的微生物群（保持稳定），并在第 24 周得以维持。与 FT 婴儿相比，早产儿 (PT) 在第 1 周的肠道微生物群显示出显着更高的变形菌丰度 (p< 0.001)。第 4 周的尿液代谢组学分析表明 PT-CS 婴儿与 FT（CS 和 SVD）婴儿相比，功能代谢谱不同。协同惯性分析显示婴儿的尿液代谢组和粪便微生物群之间存在共变。研究发现色氨酸和酪氨酸代谢途径以及脂肪酸和胆汁酸代谢受到分娩方式和胎龄的影响。这些发现证实，分娩方式和胎龄都对早期新生儿微生物群组成有显着影响。 FT 和 PT 婴儿的代谢物谱也存在显着差异。研究表明，延长母乳喂养对 24 周龄 FT-CS 婴儿的微生物群组成有显着影响，但有趣的是，对 FT-SVD 婴儿的微生物群组成没有显着影响。双胞胎之间的微生物群比两个随机婴儿之间的微生物群更相似，反映了宿主遗传学和环境的相似性对微生物群的影响。本文的在线版本 (doi:10.1186/s40168-016-0213-y) 包含补充材料，可供授权用户使用。
The gut is the most extensively studied niche of the human microbiome. The aim of this study was to characterise the initial gut microbiota development of a cohort of breastfed infants (n = 192) from 1 to 24 weeks of age. V4-V5 region 16S rRNA amplicon Illumina sequencing and, in parallel, bacteriological culture. The metabolomic profile of infant urine at 4 weeks of age was also examined by LC-MS. Full-term (FT), spontaneous vaginally delivered (SVD) infants’ microbiota remained stable at both phylum and genus levels during the 24-week period examined. FT Caesarean section (CS) infants displayed an increased faecal abundance of Firmicutes (p < 0.01) and lower abundance of Actinobacteria (p < 0.001) after the first week of life compared to FT-SVD infants. FT-CS infants gradually progressed to harbouring a microbiota closely resembling FT-SVD (which remained stable) by week 8 of life, which was maintained at week 24. The gut microbiota of preterm (PT) infants displayed a significantly greater abundance of Proteobacteria compared to FT infants (p < 0.001) at week 1. Metabolomic analysis of urine at week 4 indicated PT-CS infants have a functionally different metabolite profile than FT (both CS and SVD) infants. Co-inertia analysis showed co-variation between the urine metabolome and the faecal microbiota of the infants. Tryptophan and tyrosine metabolic pathways, as well as fatty acid and bile acid metabolism, were found to be affected by delivery mode and gestational age. These findings confirm that mode of delivery and gestational age both have significant effects on early neonatal microbiota composition. There is also a significant difference between the metabolite profile of FT and PT infants. Prolonged breastfeeding was shown to have a significant effect on the microbiota composition of FT-CS infants at 24 weeks of age, but interestingly not on that of FT-SVD infants. Twins had more similar microbiota to one another than between two random infants, reflecting the influence of similarities in both host genetics and the environment on the microbiota. The online version of this article (doi:10.1186/s40168-016-0213-y) contains supplementary material, which is available to authorized users.