Metal-ion-center assembly of ferredoxin and plastocyanin in isolated chloroplasts.
Metal-ion-center assembly of ferredoxin and plastocyanin in isolated chloroplasts.
复制标题
分离叶绿体中铁氧还蛋白和质体蓝蛋白的金属离子中心组装。
DOI：
10.1073/pnas.87.17.6748
复制
发表时间：
1990
影响因子：
11.1
通讯作者：
K. Keegstra
中科院分区：
文献类型：
--
作者：
H. Li;S. Theg;C. Bauerle;K. Keegstra
Most chloroplastic proteins are cytosolically synthesized and posttranslationally transported to their proper locations. Two examples of this group of proteins are ferredoxin and plastocyanin, both of which are metal-containing components of the photosynthetic electron-transport chain. The import process for these two proteins includes the insertion of the metal ions to produce the holo forms of the proteins. We show here that in vitro translated precursor proteins of ferredoxin and plastocyanin are synthesized as apo forms and are assembled into their respective holo forms after being imported into isolated chloroplasts. We also provide evidence that only mature-sized proteins are competent to be assembled into holo forms.
