Fluorescent Polymer Dots for Tracking SKOV3 Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy
Fluorescent Polymer Dots for Tracking SKOV3 Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy
复制标题
使用基于探针的共焦激光内镜追踪活体小鼠中的荧光聚合物点 SKOV3 细胞
DOI：
10.1007/s11307-019-01343-4
复制
发表时间：
2019-12-01
影响因子：
3.1
通讯作者：
Xiong, Liqin
中科院分区：
文献类型：
--
作者：
Zhang, Yufan;Li, Yao;Xiong, Liqin
目的：基于探针的共聚焦激光显微内镜（pCLE）是一种新的技术，允许实时和高分辨率的成像在体内。与传统的白色光内窥镜相比，它提供了显微图像，并增加了组织的穿透深度。本研究的目的是通过基于pCLE.Procedures的荧光聚合物点跟踪卵巢癌细胞在器官中的作用：SKOV 3-mCherry细胞与聚合物点在无血清培养基中孵育24 h。将标记的细胞通过静脉内、腹膜内和淋巴结注射给予裸鼠。用pCLE观察器官内标记细胞的荧光信号。结果：pCLE在器官血管中显示标记细胞的荧光信号。此外，在解毒器官，如脾脏和肾脏中可视化的标记细胞的积累随着时间的推移而增加。结论：在这篇文章中，我们提出了一种实时和方便的方法跟踪SKOV 3-mCherry在活的小鼠结合荧光聚合物点与pCLE。
Purpose:Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.Procedures:SKOV3-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.Results:pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.Conclusions:In this article, we present a real-time and convenient method for tracking SKOV3-mCherry in living mice by combined fluorescent polymer dots with pCLE.