Effects of lower alcohols on potassium transport and microsomal adenosine-triphosphatase activity of rat cerebral cortex.
Effects of lower alcohols on potassium transport and microsomal adenosine-triphosphatase activity of rat cerebral cortex.
复制标题
低级醇对大鼠大脑皮层钾转运和微粒体三磷酸腺苷酶活性的影响。
作者：
Y. Isreal;H. Kalant;A. E. Leblanc
1. 大鼠大脑皮层切片，在37度厌氧培养下，从初始浓度102m-当量/kg开始损失K(+)。浓度为 57m 当量/kg。 10分钟后。在随后的有氧培养中，它们以随培养基 K(+) 浓度变化的速率快速恢复 K(+)。 2. 低级脂肪醇具有相同的热力学活性，在有氧培养过程中对 K(+) 吸收产生大致相同程度的抑制。通过增加培养基中的 K(+) 含量可以减少这种抑制作用。乙醇不影响厌氧培养期间K(+)损失的速率。 3. Li(+)浓度在1-10mm时也能抑制大脑皮层切片对K(+)的吸收,抑制程度随Li(+)浓度的不同而变化。哇巴因还抑制 K(+) 的吸收。 4.相同系列的醇，在相同的热力学活性下，对脑微粒体中Na(+)、K(+)、Mg(2+)刺激的三磷酸腺苷酶活性产生相当程度的抑制。 5. 有人认为，在酒精和其他物质产生中枢神经抑制的过程中，阳离子转运的抑制是一个重要的但不是主要的机制。
1. Slices of rat cerebral cortex, incubated anaerobically at 37 degrees , lost K(+) from an initial concentration of 102m-equiv./kg. to a concentration of 57m-equiv./kg. after 10min. On subsequent aerobic incubation they regained K(+) rapidly at a rate that varied with the K(+) concentration of the medium. 2. Lower aliphatic alcohols, present at equal thermodynamic activity, produced approximately equal degrees of inhibition of K(+) uptake during the aerobic incubation. This inhibition was reduced by an increase in K(+) content of the medium. Ethanol did not affect the rate of K(+) loss during anaerobic incubation. 3. Li(+), in concentrations of 1-10mm, also inhibited K(+) uptake by brain-cortex slices, the degree of inhibition varying with the Li(+) concentration. Ouabain also inhibited K(+) uptake. 4. The same series of alcohols, at equal thermodynamic activity, produced comparable degrees of inhibition of Na(+),K(+),Mg(2+)-stimulated adenosine-triphosphatase activity in brain microsomes. 5. It is suggested that inhibition of cation transport is an important, but not a primary, mechanism in the production of central nervous depression by alcohols and other substances.