Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.
Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.
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人补体蛋白因子 B（III 类主要组织相容性复合体基因产物）的 cDNA 克隆的分离。
DOI：
10.1073/pnas.79.18.5661
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发表时间：
1982
影响因子：
11.1
通讯作者：
H. Colten
中科院分区：
文献类型：
--
作者：
D. Woods;Alexander F. MARKHAMt;A. Ricker;G. Goldberger;H. Colten
已从成人肝cDNA文库中分离出对应于主要组织相容性III类抗原（补体蛋白因子B）的cDNA克隆。通过与两种合成寡核苷酸混合物的直接杂交来鉴定大小为1.0至2.3个内切酶的克隆。选择因子B氨基酸序列的两个区域，每个区域在密码子分配中具有最小的模糊性，用于合成寡核苷酸。两个克隆的序列已部分确定。它们包含因子B的B B片段的氨基酸序列和整个3'非翻译区的编码信息。
cDNA clones corresponding to a major histocompatibility class III antigen, the complement protein factor B, have been isolated from a human adult liver cDNA library. The clones, ranging in size from 1.0 to 2.3 kilobases, were identified by direct hybridization with two synthetic oligonucleotide mixtures. Two regions of the factor B amino acid sequence, each with minimal ambiguity in codon assignment, were chosen for synthesis of the oligonucleotides. The sequences of two clones have been partially determined. They contain coding information for the amino acid sequence of the Bb fragment of factor B and the entire 3' -untranslated region.