DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum
DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum
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DOI：
10.1111/j.1365-2672.2006.02891.x
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发表时间：
2006-04-01
影响因子：
4
通讯作者：
Kleerebezem, M
中科院分区：
文献类型：
--
作者：
Bron, PA;Molenaar, D;Kleerebezem, M
目的：本研究的目的是确定在胆汁stress.Methods和结果：基于克隆的DNA微阵列的食品相关的乳酸菌WCFS 1的全局转录反应，描述了全局的转录反应植物乳杆菌WCFS 1对0.1%的猪胆汁。比较乳酸菌生长过程中获得的差异转录谱。在含有和不含胆汁的平板上的plantarum揭示了28和62个推定的基因，其中表达分别被胆汁上调或下调至少2.5倍。大约50%的这些基因出现遗传连锁，并确定了12个胆汁反应基因簇。七个已确定的胆汁响应基因和基因簇编码典型的应激相关功能，包括谷胱甘肽还原酶和谷氨酸脱羧酶，分别参与氧化和酸应激。此外，还鉴定了14个胆汁应答基因和基因簇，它们编码位于细胞包膜中的蛋白质，包括dlt操纵子和F1 F0 ATPase.Conclusions：鉴定了相对大量的编码细胞包膜功能的基因，表明胆汁酸对细胞质膜和细胞壁的完整性和/或功能性有重要影响。这里提出的数据提供了有价值的线索，对防御机制发挥作用，在胆汁压力在乳酸菌。植物
Aims: The purpose of this study was to determine the global transcriptional response in a food-associated lactic acid bacterium during bile stress.Methods and Results: Clone-based DNA micro-arrays were employed to describe the global transcriptional response of Lactobacillus plantarum WCFS1 towards 0.1% porcine bile. Comparison of differential transcript profiles obtained during growth of Lact. plantarum on plates with and without bile revealed 28 and 62 putative genes, of which the expression was at least 2.5-fold up- or down-regulated by bile, respectively. Approximately, 50% of these genes appeared genetically linked, and 12 bile-responsive gene clusters were identified. Seven of the identified bile-responsive genes and gene clusters encode typical stress-related functions, including glutathione reductase and glutamate decarboxylase, involved in oxidative and acid stress, respectively. Moreover, 14 bile-responsive genes and gene clusters were identified that encode proteins that are located in the cell envelope, including the dlt operon and the F1F0 ATPase.Conclusions: The identification of a relatively high number of genes encoding cell envelope functions indicates a major impact of bile acids on the integrity and/or functionality of the cytoplasmic membrane and cell wall.Significance and Impact of the Study: The data presented here provide valuable clues towards the defence mechanisms that play a role during bile stress in Lact. plantarum.