From molecules to management: Adopting DNA-based methods for monitoring biological invasions in aquatic environments
From molecules to management: Adopting DNA-based methods for monitoring biological invasions in aquatic environments
复制标题
DOI：
10.1016/j.envres.2011.02.001
复制
发表时间：
2011-10-01
影响因子：
8.3
通讯作者：
Mahon, Andrew R.
中科院分区：
文献类型：
--
作者：
Darling, John A.;Mahon, Andrew R.
最近的技术进步推动了基于DNA的方法的快速发展，这些方法旨在促进对水生环境中入侵物种的检测和监测。这些工具有望通过提高检测灵敏度、减少分析周转时间和监测成本以及提高目标识别的特异性来改进传统的监测方法。然而，尽管基于DNA的监测方法前景看好，但在决策框架中采用这些工具仍然具有挑战性。在这里，而不是探讨方法开发的技术方面，我们研究的障碍，有效地将这些方法转化为管理环境。除了调查目前使用的DNA为基础的工具，水生入侵物种监测，我们探讨与分子技术和限制的不确定性和有效地沟通其对决策的影响的可能性相关的不确定性的潜在来源。我们特别注意最近通过的基于DNA的方法检测入侵亚洲鲤鱼物种在美国五大湖地区，因为这个例子说明了许多与应用分子工具，以实现预期的管理成果相关的挑战。我们的目标是提供一个有用的评估与整合DNA为基础的方法到水生入侵物种管理的障碍，并提供建议，为今后的努力，旨在克服这些障碍。爱思唯尔公司出版
Recent technological advances have driven rapid development of DNA-based methods designed to facilitate detection and monitoring of invasive species in aquatic environments. These tools promise to improve on traditional monitoring approaches by enhancing detection sensitivity, reducing analytical turnaround times and monitoring costs, and increasing specificity of target identifications. However, despite the promise of DNA-based monitoring methods, the adoption of these tools in decision-making frameworks remains challenging. Here, rather than explore technical aspects of method development, we examine impediments to effective translation of those methods into management contexts. In addition to surveying current use of DNA-based tools for aquatic invasive species monitoring, we explore potential sources of uncertainty associated with molecular technologies and possibilities for limiting that uncertainty and effectively communicating its implications for decision-making. We pay particular attention to the recent adoption of DNA-based methods for detection of invasive Asian carp species in the United States Great Lakes region, as this example illustrates many of the challenges associated with applying molecular tools to achieve desired management outcomes. Our goal is to provide a useful assessment of the obstacles associated with integrating DNA-based methods into aquatic invasive species management, and to offer recommendations for future efforts aimed at overcoming those obstacles. Published by Elsevier Inc.