Expression hierarchy in the Yersinia type III secretion system established through YopD recognition of RNA.
Expression hierarchy in the Yersinia type III secretion system established through YopD recognition of RNA.
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DOI：
10.1111/j.1365-2958.2011.07623.x
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发表时间：
2011-05
影响因子：
3.6
通讯作者：
Anderson DM
中科院分区：
文献类型：
--
作者：
Chen Y;Anderson DM
耶尔森菌III型分泌系统（T3SS）具有环境响应性，能够在与宿主细胞接触后迅速诱导，是耶尔森菌建立复制生态位和致病的必要条件。YopD是一种转运蛋白，它抑制T3SS基因的表达，直到受到环境信号的信号，这种机制被称为低钙反应。在这项工作中，我们研究了鼠疫耶尔森菌YopD对靶基因的识别。在一个yopD突变体中，T3SS的所有基因都被诱导表达，但程度不同，以效应Yops受影响最大。第二，靶基因起始密码子上下游的短au富序列元件对于YopD介导的抑制是必要的，但不是充分的。纯化后的YopD-LcrH在体外特异性结合不同相对亲和力的靶rna，效应Yops具有更大的亲和力。这些数据表明，YopD与T3SS转录本结合，在那里它可能阻止核糖体结合，从而加速mRNA的降解。这种调节机制可以确保低钙反应期间的表达层次，因为低亲和力的yopd靶标（如伴侣蛋白）会在高亲和力靶标（如效应蛋白）之前被翻译，从而使细菌在感染期间对Yop易位进行另一层控制。
The Yersinia type III secretion system (T3SS) is environmentally responsive to enable its rapid induction upon contact with host cells and is necessary for Yersiniae to establish a replicative niche and cause disease. YopD, a translocator protein, represses the expression of T3SS genes until signaled by environmental cues, a mechanism known as the low calcium response. In this work, we investigated recognition of target genes by Yersinia pestis YopD. Expression of all genes of the T3SS was induced in a yopD mutant, though not to the same degree, with effector Yops most affected. Two, short AU-rich sequence elements up- and downstream of start codons of target genes were necessary but not sufficient for YopD mediated repression. Purified YopD-LcrH bound specifically to target RNAs in vitro with different relative affinities, with effector Yops having greater affinity. Together the data suggest YopD binds to T3SS transcripts where it may prevent ribosome binding causing accelerated mRNA degradation. This regulatory mechanism may ensure an expression hierarchy during the low calcium response as low affinity YopD-targets such as chaperones would be translated prior to high affinity targets such as effector Yops allowing the bacteria another layer of control over Yop translocation during infection.
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影响因子：
3.2
作者：
Bjornfot, Ann-Catrin;Lavander, Moa;Wolf-Watz, Hans
通讯作者：
Wolf-Watz, Hans
影响因子：
3.2
作者：
Cambronne, ED;Sorg, JA;Schneewind, O
通讯作者：
Schneewind, O
影响因子：
48
作者：
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通讯作者：
Schweizer, HP
影响因子：
3.2
作者：
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通讯作者：
Plano, GV
影响因子：
3.1
作者：
DONNENBERG, MS;KAPER, JB
通讯作者：
KAPER, JB