Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System
Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System
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DOI：
10.1177/0009922820920936
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发表时间：
2020-05-19
影响因子：
1.6
通讯作者：
Wald, Ellen R.
中科院分区：
文献类型：
--
作者：
DeMuri, Gregory;Wald, Ellen R.
快速周转的实时聚合酶链反应 (PCR) 最近已成为针对患有咽炎的儿童的 A 族链球菌 (GAS) 的即时检测。我们在这项试点研究中的目的是确定是否可以通过儿童像吸棒棒糖一样吸吮咽喉痛儿童的唾液中的拭子来检测 GAS。 20 名咽拭子 GAS 快速抗原检测呈阳性的儿童被纳入研究。使用 cobas Liat 系统对咽部和棒棒糖样本进行 PCR 检测。 20份咽拭子全部呈阳性； 20 个棒棒糖样本中有 19 个呈阳性。新型 GAS PCR 试剂盒灵敏度的提高可能允许使用侵入性更小、更舒适的采样技术进行诊断。
Rapid turnaround real-time polymerase chain reaction (PCR) has recently become available as a point-of-care test for group A Streptococcus (GAS) in children presenting with pharyngitis. Our aim in this pilot study was to determine if GAS can be detected in the saliva of children with sore throat using swabs inoculated by children sucking on them as they would a lollipop. Twenty children with positive rapid antigen detection tests for GAS from pharyngeal swabs were enrolled. Pharyngeal and lollipop samples underwent PCR testing using the cobas Liat system. All 20 pharyngeal swabs were positive; 19 of 20 lollipop samples were positive. The increased sensitivity of the new PCR kits for GAS may permit use of less invasive and more comfortable sampling techniques for diagnosis.