RELATIONSHIP BETWEEN PYOCINS AND A BACTERIOPHAGE IN PSEUDOMONAS AERUGINOSA
RELATIONSHIP BETWEEN PYOCINS AND A BACTERIOPHAGE IN PSEUDOMONAS AERUGINOSA
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铜绿假单胞菌中脓毒菌素与噬菌体之间的关系
DOI：
10.2323/jgam.16.3_231
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发表时间：
1970
影响因子：
1.2
通讯作者：
M. Kageyama
中科院分区：
文献类型：
--
作者：
S. Ito;M. Kageyama
从铜绿假单胞菌的溶原性菌株中分离到一株与R型绿脓菌素相关的噬菌体，命名为PS3。它由一个头部和一个可收缩的尾部组成，类似于R型绿脓菌素。与R型绿脓菌素进行了比较，发现该噬菌体的活性可被抗R和R2血清中和，抗PS 3的抗血清对R和R2也有抑制作用。然而，抗R鞘蛋白血清对绿脓菌素R或R2有很强的中和活性，而对噬菌体PS 3几乎无效。对其中一种杀伤粒子具有抗性的突变体被分离出来，并研究了它们对其他药剂的行为。根据这一结果，提出了关于噬菌体和绿脓菌素受体结构的工作假设。
A bacteriophage related to R-type pyocins was isolated from a lysogenic strain of Pseudomonas aeruginosa and named PS3. It was composed of a head and a contractile tail which resembled R-type pyocins. The character of the phage was studied comparing with R-type pyocins.The activity of this phage was neutralized by anti-R or R2 serum and the antiserum against PS3 was also active in inhibiting pyocins R and R2. However, the anti-R sheath serum, while showing a strong neutralizing activity against pyocin R or R2, was almost ineffective against the phage PS3.The phage and the pyocins were further related in some way in the receptor specificity. Mutants resistant to one of the killer particles were isolated and their behavior to other agents were studied. From this result, a working hypothesis was presented with regard to the structure of the receptor for the phage and pyocins.