Blood group a antigen is a coreceptor in Plasmodium falciparum resetting
Blood group a antigen is a coreceptor in Plasmodium falciparum resetting
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DOI：
10.1128/iai.68.5.2971-2975.2000
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发表时间：
2000-05-01
影响因子：
3.1
通讯作者：
Carlson, J
中科院分区：
文献类型：
--
作者：
Barragan, A;Kremsner, PG;Carlson, J
疟疾寄生虫恶性疟原虫利用存在于未感染的红细胞（RBC）表面上的分子形成玫瑰花结，并且先前已经显示出对ABO抗原的依赖性。免疫血清的抗玫瑰花结作用与宿主血型有关。来自疟疾免疫血型A（或B）个体的血清不太容易破坏来自血型A（或B）患者的临床分离株的RORISK，而不是破坏来自血型O患者的分离株的RORISK。所有新鲜的临床分离株和实验室菌株均表现出明显的ABO血型偏好，表明血型抗原的利用是恶性疟原虫重置的一般特征。可溶性A抗原强烈抑制玫瑰花结的形成时，寄生虫培养在A红细胞，而抑制糖胺聚糖下降。此外，可溶性A抗原结合物结合到寄生的RBC的细胞表面。选择性酶消化未感染的红细胞表面的A型抗原，完全消除了寄生虫与这些红细胞形成罗丹明的偏好，但罗丹明仍然可以形成。总之，目前的数据表明，A和B抗原作为辅助受体在恶性疟原虫复位中的重要作用。
The malaria parasite Plasmodium falciparum utilizes molecules present on the surface of uninfected red blood cells (RBC) for rosette formation, and a dependency on ABO antigens has been previously shown. In this study, the antirosetting effect of immune sera was related to the blood group of the infected human host. Sera from malaria-immune blood group A (or B) individuals were less prone to disrupt rosettes from clinical isolates of blood group A (or B) patients than to disrupt rosettes from isolates of blood group O patients. All fresh clinical isolates and laboratory strains exhibited distinct ABO blood group preferences, indicating that utilization of blood group antigens is a general feature of P. falciparum resetting. Soluble A antigen strongly inhibited rosette formation when the parasite was cultivated in A RBC, while inhibition by glycosaminoglycans decreased. Furthermore, a soluble A antigen conjugate bound to the cell surface of parasitized RBC. Selective enzymatic digestion of blood group A antigen from the uninfected RBC surfaces totally abolished the preference of the parasite to form rosettes with these RBC, but rosettes could still form. Altogether, present data suggest an important role for A and B antigens as coreceptors in P. falciparum resetting.