DEUTERIUM AND O-18 MEASUREMENTS ON MICROLITER SAMPLES OF URINE, PLASMA, SALIVA, AND HUMAN-MILK
DEUTERIUM AND O-18 MEASUREMENTS ON MICROLITER SAMPLES OF URINE, PLASMA, SALIVA, AND HUMAN-MILK
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DOI：
10.1093/ajcn/45.5.905
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发表时间：
1987-05-01
影响因子：
7.1
通讯作者：
KLEIN, PD
中科院分区：
文献类型：
--
作者：
WONG, WW;LEE, LS;KLEIN, PD
本文介绍了在微升生物体液样品上测量2 H：1H和18 O：16 O同位素比值的改进方法。2 H的富集水平（580.permill.）和18 O（256.permill.）在尿、血浆、唾液和人乳中的浓度可以以3.2.permill的精度测量。（n=200）和0.97%。（n=200），准确度为-4.6 ±-。4.4.permill. (mean .+-. SD，n=200）和-0.32 . ±. 0.87.permill. (mean .+-. SD，n=200）。在石英反应容器中通过锌还原由10 μ L未蒸馏流体产生氢气样品。使用改进的商业平衡系统在10小时内完成用于18 O测量的100 μ L样品的水-CO2平衡。这些方法上的改进促进和扩展了2 H和18 O示踪剂在人体成分和能量消耗研究中的应用。
Improved methods to measure 2H:1H and 18O:16O isotope ratios on microliter samples of biological fluids are described. Enriched levels of 2H (580.permill.) and 18O (256.permill.) in urine, plasma, saliva, and human milk can be measured with a precision of 3.2.permill. (n=200) and 0.97.permill. (n=200) and an accuracy of -4.6 .+-. 4.4.permill. (mean .+-. SD, n=200) and -0.32 .+-. 0.87.permill. (mean .+-. SD, n=200), respectively. Hydrogen gas samples are generated from 10 .mu.L of undistilled fluid by zinc reduction in quartz reaction vessels. Water-CO2 equilibration of a 100-.mu.L sample for 18O measurement is completed in 10 h using a modified commercial equilibration system. These methodological improvements facilitate and extend the use of 2H and 18O tracers in studies of body composition and energy expenditure.