Analysis of pupal head proteome and its alteration in diapausing pupae of Helicoverpa armigera
Analysis of pupal head proteome and its alteration in diapausing pupae of Helicoverpa armigera
复制标题
棉铃虫滞育蛹蛹头蛋白质组及其变化分析
DOI：
10.1016/j.jinsphys.2009.10.008
复制
发表时间：
2010-03-01
影响因子：
2.2
通讯作者：
Lei, Chaoliang
中科院分区：
文献类型：
--
作者：
Chen, Lizhen;Ma, Weihua;Lei, Chaoliang
蛋白质组学方法已被证明是了解昆虫滞育过程的有用工具。利用二维凝胶电泳和基质辅助激光解吸电离（MALDI）飞行时间（TOF）技术，从棉铃虫头部蛋白质组中鉴定出24个具有不同功能特性的蛋白质，包括细胞骨架蛋白、热休克蛋白、昆虫发育调节因子、ATP酶、信号通路调节蛋白和代谢相关酶，非滞育蛹和滞育蛹之间的蛋白质组学比较揭示了仅存在于非滞育蛹中的三种蛋白质，并且六种蛋白质代表大于等于2.0倍或大于等于2.0倍的蛋白质。
The proteomic approach has proven to be an useful tool for understanding insect diapause processes. Using 2D gel electrophoresis and matrix assisted laser/desorption ionization (MALDI) time of flight (TOF), we identified 24 proteins in the head of Helicoverpa armigera pupae with diverse functional characteristics, including cytoskeleton proteins, heat-shock proteins, insect development regulation factors, ATPases, proteins regulating signal pathway and enzymes involved in metabolism, etc. A proteomic comparison between nondiapausing and diapausing pupae revealed three proteins that were present only in nondiapausing pupae, and six proteins represented >= 2.0-fold or