Pulmonary Endodermal Tumor Resembling Fetal Lung: The Optically Clear Nucleus Is Rich in Biotin
Pulmonary Endodermal Tumor Resembling Fetal Lung: The Optically Clear Nucleus Is Rich in Biotin
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类似胎肺的肺内胚层肿瘤：光学透明的细胞核富含生物素
DOI：
10.1097/00000478-199406000-00008
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
N. Ogawa
中科院分区：
文献类型：
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作者：
Y. Nakatani;H. Kitamura;Y. Inayama;N. Ogawa
肺内胚层瘤类似胎儿肺（PET）是一种罕见的肺部肿瘤，可能是一个独特的形式，肺母细胞瘤缺乏肉瘤的组成部分。PET的一个独特的组织学特征是在桑椹区存在光学透明核（OCN），其通常通过抗生物素蛋白-生物素过氧化物酶复合物（ABC）方法显示假阳性免疫染色。由于最近的研究表明，在妊娠期间卵巢类性腺癌和子宫内膜腺上皮中所见的相同变化的细胞核富含生物素，我们进行了一项调查，以了解发生在一名45岁男子身上的PET是否也是如此。当组织切片与过氧化物酶孵育时，只有OCN被阳性染色，标记的抗生物素蛋白或链霉抗生物素蛋白并在色原溶液中显影。用游离抗生物素蛋白预处理可完全阻断反应。当采用抗生素抗体作为主要试剂时，通过间接免疫过氧化物酶法观察到相同的核染色模式。肿瘤细胞的雌激素和孕激素受体的免疫组化染色阴性的冷冻组织切片。在超微结构上，OCN被直径约为10至12 nm的染色质丝状亚结构占据。这些结果表明，PET中的OCN是体内某些肿瘤和非肿瘤细胞独特核变化的一部分，其中丰富的生物素在细胞核内积累，与特征性排列的染色质亚结构相关。进一步的研究是必要的，以确定OCN是否通常在女性性激素的影响下发展。当在PET和某些其他上皮病变中使用ABC方法或相关技术进行免疫染色时，应牢记OCN中假阳性的可能性，以避免对结果的任何误解。
Pulmonary endodermal tumor resembling fetal lung (PET) is a rare pulmonary neoplasm that may represent a distinctive form of pulmonary blastoma lacking the sarcomatous component. A peculiar histologic feature of PET is the presence in the morular area of optically clear nuclei (OCN), which commonly show false-positive immunostaining by the avidin-biotin peroxidase complex (ABC) method. Because recent studies have shown that the nuclei of an identical change seen in endometrioid adenocarcinoma of the ovary and endometrial glandular epithelium during pregnancy are rich in biotin, we conducted an investigation to see if the same was true of a PET occurring in a 45-year-old man. Only the OCN were positively stained when the tissue section was incubated with peroxidase-labeled avidin or streptavidin and developed in a chromogen solution. The reaction was completely blocked by pretreatment with free avidin. An identical nuclear staining pattern was observed when the antibiotin antibody was applied as the primary agent by the indirect immunoperoxidase method. The neoplastic cells were negative for estrogen and progesterone receptors in immunostaining on frozen tissue sections. Ultra-structurally, the OCN were occupied by filamentous substructures of chromatin measuring approximately 10 to 12 nm in diameter. These results suggest that the OCN in PET are part of the unique nuclear change of certain neoplastic and non-neoplastic cells in the body in which abundant biotin accumulates within the nucleus in association with the characteristically arranged chromatin substructure. Further study is necessary to determine whether the OCN generally develop under the influence of female sex hormones. When immunostaining by the ABC method or related techniques is performed in PET and certain other epithelial lesions, the possibility of false positivity in the OCN should be kept in mind to avoid any misinterpretation of the results.