Tissue drug concentration determines whether fluorescence or absorption measurements are more sensitive in diffuse optical tomography of exogenous contrast agents
Tissue drug concentration determines whether fluorescence or absorption measurements are more sensitive in diffuse optical tomography of exogenous contrast agents
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DOI：
10.1364/ao.48.00d262
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发表时间：
2009-04-01
期刊：
影响因子：
1.9
通讯作者：
Paulsen, Keith D.
中科院分区：
文献类型：
--
作者：
Davis, Scott C.;Pogue, Brian W.;Paulsen, Keith D.
比较组织中吸收和发荧光的物体的测量灵敏度，以确定荧光数据优于从吸收得到的荧光数据的条件。使用模拟的人类乳房体积来模拟边界数据中的相对扰动，所述相对扰动由包含高浓度的理论外源性造影剂的深埋异常引起，所述理论外源性造影剂具有类似于德克萨斯卟啉镥（LuTex）和吲哚菁绿色（ICG）的吸收特性。合成数据用于产生量子产率值，其表示有利于荧光与吸收成像的条件之间的转变。探索的参数包括肿瘤背景对比度、背景药物浓度和激发光过滤效率。背景中的药物浓度是确定哪种对比机制提供更灵敏测量的主要因素。具体地，如果背景药物浓度对于LuTex低于135-200 nM并且对于ICG低于25-50 nM，则荧光测量是有利的，而吸收测量在这些范围以上更敏感。(C)2009年美国光学学会
The measurement sensitivities of absorbing and fluorescing objects in tissue are compared to determine conditions for which fluorescence data are favorable over those derived from absorption. A simulated human breast volume was used to model the relative perturbation in boundary data caused by a deeply embedded anomaly containing elevated concentrations of theoretical exogenous contrast agents with absorption properties resembling lutetium texaphyrin (LuTex) and Indocyanine Green (ICG). Synthetic data were used to produce quantum yield values representing the transition between conditions favorable to fluorescence versus absorption imaging. The parameters explored include tumor-to-background contrast, background drug concentration, and excitation light filtering efficiency. Drug concentration in the background was the primary factor that determined which contrast mechanism provided the more sensitive measurements. Specifically, fluorescence measurements are favorable if background drug concentrations are below 135-200 nM for LuTex and 25-50 nM for ICG, while absorption measurements are more sensitive above these ranges. (C) 2009 Optical Society of America