Growth inhibitory effect of sodium azide in chemosensitivity assays.
Growth inhibitory effect of sodium azide in chemosensitivity assays.
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叠氮化钠在化学敏感性测定中的生长抑制作用。
DOI：
10.1002/stem.5530050303
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Kern,DH
中科院分区：
文献类型：
--
作者：
Sondak,VK;Hildebrand-Zanki,SU;Butler,A;Kern,DH
基于菌落计数的体外化疗敏感性试验受到假阴性结果持续发生率的困扰。为了避免严重的预测错误，一些研究者使用阳性对照（已知的有毒化合物）作为质量控制措施。在17/35份（49%）试验中，6 mg/ml叠氮化钠（NaN 3）未能抑制菌落形成;而在胸苷掺入试验中，叠氮化钠可有效抑制124/131份（95%）标本。必须仔细选择用于化学敏感性试验的阳性对照物质，以确保结果准确。
In vitro chemosensitivity assays based on colony counting are plagued by persistent incidence of false‐negative results. To avoid serious predictive errors, some investigators have utilized positive controls (known toxic compounds) as a quality control measure. Sodium azide (NaN3) at 6 mg/ml failed to inhibit colony formation in 17/35 (49%) assays; while in the thymidine incorporation assay, sodium azide was effective in inhibiting 124/131 (95%) specimens. Positive control substances for use in chemosensitivity assays must be carefully selected to insure accurate results.