Nucleotide sequence of the VH, VL regions of an anti-idiotopic antibody reacting with a private idiotope of the anti-lysozyme D1.3 antibody.
Nucleotide sequence of the VH, VL regions of an anti-idiotopic antibody reacting with a private idiotope of the anti-lysozyme D1.3 antibody.
复制标题
与抗溶菌酶D1.3抗体的私有独特位反应的抗独特位抗体的VH、VL区的核苷酸序列。
DOI：
10.1016/0161-5890(90)90167-x
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
Poljak,RJ
中科院分区：
文献类型：
--
作者：
Souchon,H;Doyen,N;Riottot,MM;Rougeon,F;Poljak,RJ
抗体E225与抗溶菌酶抗体D1.3的私有独特表位反应。从这些BALB/c单克隆抗体的Fab片段之间的复合物已结晶和测定的三维结构的独特位-抗独特位复合物正在进行中。这里提出的E225的核苷酸VHand VL序列已被确定，以提供必要的氨基酸序列信息的解释的高分辨率的电子密度图的复杂的，通过X-射线晶体学。将从VK和VHmRNA合成的cDNA克隆到E.杆菌通过双脱氧终止法对两条cDNA链进行测序。翻译的氨基酸序列显示VK、VH分别对应于小鼠免疫球蛋白轻链和重链的组五（V）和组II（B）。E225的互补决定区和D1.3识别的溶菌酶抗原决定簇之间的序列比对不能表明抗独特型抗体是否在结构上模拟外部抗原。
Antibody E225 reacts with a private idiotope of the anti-lysozyme antibody D1.3. A complex between the Fab fragments from these BALB/c monoclonal antibodies has been crystallized and the determination of the three-dimensional structure of this idiotope-anti-idiotope complex is under way. The nucleotide VHand VLsequences of E225 presented here have been determined to provide the amino acid sequence information necessary for the interpretation of the high resolution electron density maps of the complex, obtained by X-ray crystallography. The cDNAs synthesized from the VKand VHmRNAs were cloned inE. coli. Both cDNA strands were sequenced by the dideoxy termination method. The translated amino acid sequence shows that VK, VHcorrespond to groups five (V) and II(b) of mouse immunoglobulin light and heavy chains, respectively. Sequence alignments between the complementarity determining regions of E225 and the antigenic determinant of lysozyme recognized by D 1.3 do not indicate whether or not the anti-idiotoplc antibody structurally mimics the external antigen.