Serotonin Regulates the Feeding and Reproductive Behaviors of Pratylenchus penetrans.
Serotonin Regulates the Feeding and Reproductive Behaviors of Pratylenchus penetrans.
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DOI：
10.1094/phyto-11-16-0397-r
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发表时间：
2017-07
期刊：
影响因子：
3.2
通讯作者：
Schroeder NE
中科院分区：
文献类型：
--
作者：
Han Z;Boas S;Schroeder NE
所有植物寄生线虫的成功都取决于几种复杂行为的完成。短体线虫（Pratylenchus）是一种重要的经济寄生虫，寄生于多种植物宿主。与孢囊线虫和根结线虫不同的是，P. paclitrans在宿主根的内部和外部都可以移动，并且可以从两个位置进食。雌虫需要通过主动移动雄虫进行授精来繁殖，并且可以在寄主根内外产卵。我们对这些行为的分子基础还没有完全的了解。这些行为的一个候选调节剂是神经递质血清素。以往的研究表明，外源性5-羟色胺的影响，囊线虫和根结线虫的取食和雄性交配行为。然而，没有关于外源5-羟色胺对病变线虫的作用的数据。同样，也没有数据的存在和功能的内源性血清素在任何植物寄生线虫。在这里，我们建立了外源性5-羟色胺应用于P. pastrans调节喂养和性别特异性行为。此外，使用免疫组织化学和药理学测定，我们的数据表明，P. pastrans利用内源性5-羟色胺来调节摄食和性别特异性行为。
The success of all plant-parasitic nematodes is dependent on the completion of several complex behaviors. The lesion nematode Pratylenchus penetrans is an economically important parasite of a diverse range of plant hosts. Unlike the cyst and root-knot nematodes, P. penetrans moves both within and outside of the host roots and can feed from both locations. Adult females of P. penetrans require insemination by actively moving males for reproduction and can lay eggs both within and outside of the host roots. We do not have a complete understanding of the molecular basis for these behaviors. One candidate modulator of these behaviors is the neurotransmitter serotonin. Previous research demonstrated an effect of exogenously applied serotonin on the feeding and male mating behaviors of cyst and root-knot nematodes. However, there are no data on the role of exogenous serotonin on lesion nematodes. Similarly, there are no data on the presence and function of endogenous serotonin in any plant-parasitic nematode. Here, we establish that exogenous serotonin applied to P. penetrans regulates both feeding and sex-specific behaviors. Furthermore, using immunohistochemistry and pharmacological assays, our data suggest that P. penetrans utilizes endogenous serotonin to regulate both feeding and sex-specific behaviors.